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SN/T 5334.2-2020: (Digital PCR Detection Method of Genetically Modified Plant Products - Part 2: Genetically Modified Soybean)
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 5334.2-2020 | English | 179 |
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(Digital PCR Detection Method of Genetically Modified Plant Products - Part 2: Genetically Modified Soybean)
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SN/T 5334.2-2020
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Basic data | Standard ID | SN/T 5334.2-2020 (SN/T5334.2-2020) | | Description (Translated English) | (Digital PCR Detection Method of Genetically Modified Plant Products - Part 2: Genetically Modified Soybean) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 8,857 | | Date of Issue | 2020-12-30 | | Date of Implementation | 2021-07-01 | | Regulation (derived from) | General Administration of Customs Announcement No. 136 [2020] | | Issuing agency(ies) | General Administration of Customs | SN/T 5334.2-2020: (Digital PCR Detection Method of Genetically Modified Plant Products - Part 2: Genetically Modified Soybean)
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards
2020-12-30 release
2021-07-01 implementation
Issued by the General Administration of Customs of the People's Republic of China
Foreword
SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts.
--Part 1.General requirements and definitions;
--Part 2.Genetically modified soybeans;
--Part 3.Genetically modified corn;
--Part 4.Genetically modified rape;
--Part 5.Genetically modified cotton;
--Part 6.Genetically Modified Potatoes;
--Part 7.Genetically modified alfalfa;
--Part 8.Genetically modified sugar beet.
This document is part 2 of SN/T 5334-2020.
This document was drafted in accordance with the rules given in GB/T 1.1-2020.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.
This document was proposed and managed by the General Administration of Customs of the People's Republic of China.
Drafting organizations of this document. Chinese Academy of Inspection and Quarantine, Dalian Customs Technology Center of the People’s Republic of China,
Heguo Shenyang Customs Technology Center, Guangzhou Customs Technology Center of the People’s Republic of China, Qingdao Customs Technology Center of the People’s Republic of China, China
Shenzhen Customs Food Inspection and Quarantine Technology Center of the People's Republic of China, Shanghai Customs of the People's Republic of China.
The main drafters of this document. Cao Jijuan, Zheng Qiuyue, Xiao Xizhi, Liu Jin, Zhu Pengyu, Fu Wei, Yang Lili, Xu Junyi.
Digital PCR detection method for genetically modified plant products
Part 2.Genetically Modified Soy
1 Scope
This document specifies the quantitative detection method of genetically modified strains specific to imported and exported soybeans by digital PCR (dPCR).
This document is applicable to the quantitative detection of genetically modified components in soybean seeds and leaves. See Appendix A for the detection limits of this document.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable
Used in this document.
SN/T 5334.1 Digital PCR Method for Quantitative Detection of Genetically Modified Products Part 1.General Requirements and Definitions
3 Terms and definitions
The terms and definitions defined in SN/T 5334.1 apply to this document.
4 Principles of the method
See SN/T 5334.1.
5 Main equipment and reagents
5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.
6 Method operation steps
6.1 General operation steps
For general operation steps, see SN/T 5334.1.
6.2 Digital PCR reaction system
See Table 1 for the preparation of the digital PCR reaction system.
6.3 Digital PCR reaction program
7 Judgment and expression of results
Refer to SN/T 5334.1 for the calculation method of the results.
The results are expressed as follows.
- In the test results, both the internal standard gene and the foreign gene meet the negative and blank quality control requirements, indicating that no soybean has been detected.
Source gene Lectin, expressed as "no soybean component detected";
- In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said
Genetic strain";
- In the test results, both the internal standard gene and the exogenous genome meet the quality control requirements of the digital PCR test of the sample, indicating that
8 Anti-pollution measures
The anti-pollution measures are specified in SN/T 5334.1.
9 Sample storage
For sample storage, see SN/T 5334.1.
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