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DNA barcoding screening method - Part 2: Quarantine Xanthomonas spp.
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SN/T 4877.2-2017
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Basic data Standard ID | SN/T 4877.2-2017 (SN/T4877.2-2017) | Description (Translated English) | DNA barcoding screening method - Part 2: Quarantine Xanthomonas spp. | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | C62 | Word Count Estimation | 7,740 | Date of Issue | 2017-07-21 | Date of Implementation | 2018-03-01 | Quoted Standard | SN/T 1400; SN/T 2372; SN/T 3278; SN/T 3431; SN/T 4073 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the amplification, analysis and determination of results in the DNA barcode screening of Phytophthora vaccae. This standard applies to the screening of quarantine Xanthomonas. |
SN/T 4877.2-2017: DNA barcoding screening method - Part 2: Quarantine Xanthomonas spp.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
DNA barcoding screening method - Part 2. Quarantine Xanthomonas spp.
People's Republic of China entry and exit inspection and quarantine industry standards
Gene barcode screening method
Part 2. Phytophthora vaccae
Part 2. QuarantineXanthomonasspp.
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4877 "Gene Barcode Screening Method" is divided into three parts.
--- Part 1. Quarantine coryneform bacteria;
--- Part 2. Phytophthora xanthan;
--- Part 3. Quarantine phytoplasma.
This part is the second part of SN/T 4877.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. China Academy of Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people
Heguo Yunfu Entry-Exit Inspection and Quarantine Bureau, Manzhouli Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, and Institute of Biotechnology of China Academy of Agricultural Sciences.
The main drafters of this section. Tian Wei, Feng Jianjun, He Xunuo, Liu Yuqi, Zhao Wenjun, Li Weimin.
Gene barcode screening method
Part 2. Phytophthora vaccae
1 Scope
This part of SN/T 4877 specifies the amplification, analysis and determination of the sequence in the DNA barcode screening of Phytophthora vaccae.
This section applies to the screening of quarantine Xanthomonas.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
Quarantine and identification method for SN/T 1400 sugarcane gum
Method for detecting SN/T 2372 rice bacterial blight and rice bacterial leaf spot pathogen
Quarantine and identification method for SN/T 3278 hyacinth yellow rot
Quarantine and identification method for SN/T 3431 banana necrosis
SN/T 4073 rapid detection method for mango bacterial black spot disease
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 DNA barcode
A standard, sufficiently variable, easily amplified and relatively short DNA fragment of the species that can represent the species.
3.2 16SrRNA gene
A component of the 30S small subunit of the prokaryotic ribosome, the 16SrRNA gene sequence is highly conserved and specific, and is a bacterium.
An important basis for interspecific identification.
3.3 cpn60 gene
A gene encoding chaperone protein 60 (cpn60, also known as heat shock protein 60/Hsp60), a single-copy gene that is widely found in bacteria and true
Nuclear biological cells are effective molecular markers for phylogenetic analysis.
4 Xanthomonas basic information
Scientific name. Xanthomonas
Classification status. Bacteria, Proteobacteria, Gammaproteobacteria, Huangdan
Xanthomonodales, Xanthomonodaceae, Xanthomonas.
Xanthomonas is the most important genus of plant pathogenic bacteria, and the genus currently includes 14 entry phytosanitary pests.
1. Sugarcane white stripe pathogen (Xanthomonas albilineans);
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