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SN/T 4877.10-2017: DNA barcoding screening method - Part 10: quarantine Phytophthora
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DNA barcoding screening method - Part 10: quarantine Phytophthora
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SN/T 4877.10-2017
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Basic data | Standard ID | SN/T 4877.10-2017 (SN/T4877.10-2017) | | Description (Translated English) | DNA barcoding screening method - Part 10: quarantine Phytophthora | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 19,156 | | Date of Issue | 2017-08-29 | | Date of Implementation | 2018-04-01 | | Regulation (derived from) | National Quality Inspection (2017) 384 | | Issuing agency(ies) | General Administration of Customs | SN/T 4877.10-2017: DNA barcoding screening method - Part 10: quarantine Phytophthora---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Screening methods for gene barcodes - Part 10. Phytophthora)
People's Republic of China entry and exit inspection and quarantine industry standards
Gene barcode screening method
Part 10. Quarantine Phytophthora
DNAbarcodingscreeningmethod-Part 10. QuarantinePhytophthora
Published on.2017-08-29
2018-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4877 "Gene Barcode Screening Method" is divided into 10 parts.
--- Part 1. Quarantine coryneform bacteria;
--- Part 2. Phytophthora xanthan;
--- Part 3. Quarantine phytoplasma;
--- Part 4. Quarantine stem mold;
--- Part 5. Quarantine Pseudomonas spp.;
--- Part 6. Quarantine acidophilus;
--- Part 7. Phytophthora Verticillium;
--- Part 8. Quarantine anthrax;
--- Part 9. Quarantine smut;
--- Part 10. Quarantine Phytophthora.
This part is the 10th part of SN/T 4877.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine.
Drafters of this section. Zhang Guiming, Gao Ruifang, Wang Ying, Cheng Yinghui, Wang Ying, Huang Heqing.
Gene barcode screening method
Part 10. Quarantine Phytophthora
1 Scope
This part of SN/T 4877 specifies Phytophthora capsici blackwater pathogen, Phytophthora capsici rot, Phytophthora infestans, Phytophthora raspberry
Root rot, Phytophthora lucidum, Phytophthora capsici root rot, Phytophthora root rot, Phytophthora sojae, eucalyptus, and soybean disease
DNA barcode screening of mold and Phytophthora syriae.
This section applies to Phytophthora capsici blackwater pathogen, Phytophthora capsici, Phytophthora erythraea, Phytophthora capsici, root rot, citrus
Phytophthora sojae, Phytophthora capsici root rot, Phytophthora root rot, Phytophthora sojae, eucalyptus, Phytophthora sojae and clove
Amplification, analysis and determination of results of DNA barcode screening of Phytophthora infestans.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 1131 Phytophthora Phytophthora Quarantine and Identification Method
SN/T 1135.6 Quarantine and identification method for potato rot
SN/T 2080 Quarantine and identification method for eucalyptus death
Real-time fluorescent PCR detection method for SN/T 2474 Phytophthora sojae
SN/T 2617 Phytophthora Phytophthora Quarantine Identification Method
SN/T 2756 Phytophthora Phytophthora Quarantine Identification Method
SN/T 2759 Quarantine and identification method for Phytophthora capsici
SN/T 3403 Phytophthora Phytophthora Quarantine Identification Method
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNA barcode DNAbarcode
A standard, sufficiently variable, easily amplified and relatively short DNA fragment of the species that can represent the species.
3.2
Mitochondrial cytochrome c oxidase subunit I gene mitochondrialcytochromecoxidasesubunitI, COI
The rate of mitochondrial gene changes rapidly. Among the 13 protein-coding genes, there are few insertions and deletions in the COI gene, and the sequence length is 500 bp~
700bp, suitable as a fungal DNA barcode, is also the earliest proposed DNA barcode fragment.
3.3
Internal transcribed spacer sequence internaltranscribedspacer; ITS
In eukaryotes, ribosomal DNA consists of a ribosome gene and a spacer adjacent to it, with a genome sequence from 5' to 3'
In order. external transcribed spacer, 18S gene, internal transcribed spacer 1 (ITS1), 5.8S gene, internal transcribed spacer 2 (ITS2),
28S gene and gene spacer sequence. The internal transcribed spacer is the region present between 18SrDNA, 5.8SrDNA and 28SrDNA
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