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SN/T 4824-2017: Detection method for Akabane virus of bovine and ovine by LAMP
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Detection method for Akabane virus of bovine and ovine by LAMP
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Basic data
| Standard ID | SN/T 4824-2017 (SN/T4824-2017) |
| Description (Translated English) | Detection method for Akabane virus of bovine and ovine by LAMP |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Word Count Estimation | 9,976 |
| Date of Issue | 2017-07-21 |
| Date of Implementation | 2018-03-01 |
| Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4824-2017: Detection method for Akabane virus of bovine and ovine by LAMP
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Identification method of ring - mediated isothermal amplification of)
People's Republic of China entry and exit inspection and quarantine industry standards
Bovine sheep red feather disease virus loop-mediated isothermal amplification
Detection method
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, South China Agricultural University.
The main drafters of this standard. Yu Haiqiong, Cai Xianquan, Cheng Yiyi, Jia Kun, Zhao Wei, Wang Ying, Tian Chunjian, Chen Fang, Chen Ru, Lin Zhixiong.
Bovine sheep red feather disease virus loop-mediated isothermal amplification
Detection method
1 Scope
This standard specifies the ring-mediated isothermal amplification detection method for bovine and sheep red feather disease virus.
This standard is applicable to the rapid initial screening test of cattle and sheep red feather virus nucleic acid.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 18088 entry and exit animal quarantine sampling
3 Abbreviations
The following abbreviations apply to this document.
AKAV Akabanevirus
LAMP loop-mediated isothermal amplification (Loop-mediated isothermalamplification)
4 Overview of Akabane disease
The Akabies virus is a single-stranded RNA virus belonging to the genus Bunnivirus of the Bunny virus. See Appendix A for a summary of the red feather disease caused by it.
5 reagent
5.1 RNA extraction kit.
5.2 BstDNA polymerase.
5.3 Betaine Betaine.
5.4 0.01 mol/L PBS buffer (see Appendix B).
5.5 10 x Thermopol buffer.
5.6 100 mmol/L MgSO4.
5.7 10 mmol/LdNTP.
5.8 5×AMVBuffer and AMVRTase.
5.9 AKAV cell culture (inactivated).
5.10 Internal Primer (FIP BIP).
5.11 External primer (F3 B3).
5.12 RNasin.
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