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SN/T 4781-2017: Detection of Shiga toxin-producing Escherichia coli in food and animal feed for exports--Real-time PCR method
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Detection of Shiga toxin-producing Escherichia coli in food and animal feed for exports--Real-time PCR method
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SN/T 4781-2017
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Basic data | Standard ID | SN/T 4781-2017 (SN/T4781-2017) | | Description (Translated English) | Detection of Shiga toxin-producing Escherichia coli in food and animal feed for exports--Real-time PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Word Count Estimation | 18,119 | | Date of Issue | 2017-05-12 | | Date of Implementation | 2017-12-01 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 228 | | Issuing agency(ies) | General Administration of Customs | SN/T 4781-2017: Detection of Shiga toxin-producing Escherichia coli in food and animal feed for exports--Real-time PCR method
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Detection of Shiga toxin-producing Escherichia coli in food and animal feed for exports--Real-time PCR method
China's entry-exit inspection and quarantine industry standards
Shima Toxin Large Intestine Exported to Food and Feed
Escherichia coli detection method real-time fluorescence PCR
Published on.2017-05-12
2017-12-01 implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard was drafted by. Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and Shanghai Entry-Exit Inspection and Inspection
Epidemic Bureau.
The main drafters of this standard. Shao Jingdong, Shen Jinling, Feng Zhen, Wang Chuanxian, Xue Feng, Guo Yi, Wu Fuping, Wang Yiqian, Li Xiaohong, Zhu Changqing,
Xiao Zhen, Cui Siyu.
Shima Toxin Large Intestine Exported to Food and Feed
Escherichia coli detection method real-time fluorescence PCR
1 Scope
This standard specifies a method for rapid real-time PCR detection of Shiga toxin-producing Escherichia coli in food and feed.
This standard applies to Shiga toxin-producing E. coli (O157, O104, O111, O26, O145 and
O103) detection.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB 19489 General Requirements for Laboratory Biosafety
GB/T 27403 laboratory quality control standard food molecular biology test
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Shiga toxin-producing Escherichia coli Shigatoxin-producing Escherichiacoli; STEC
Escherichia coli containing Shiga toxin encoding gene stx.
3.2
Enterohaemorrhagic E. coli Shigatoxin-producingEscherichiacolicausingtheattachingand
Effacinglesion
It also contains the Shiga toxin coding gene stx and the chymoid coding gene eae.
Note. This combination of virulence genes is often associated with severe disease caused by STEC.
3.3
Real-time PCR realtime PCR
The fluorescent group was added to the PCR reaction system, and the entire PCR amplification process was monitored in real time using the accumulation of fluorescence signals.
3.4
Ct value cyclethreshold
The number of cycles experienced when the fluorescence signal within each reaction tube reached the set threshold.
4 Method summary
Nucleic acid is extracted from the microorganisms in the sample, and the Shigtoxin-encoding gene stx is tightly bound by the real-time fluorescent PCR method.
The obese encoding gene eae was detected. If stx is positive in the sample, continue the strain isolation; if stx and eae are positive, continue O blood
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