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Detection of spoilage yeasts in food for export--Real-time PCR method
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SN/T 4780-2017
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Basic data Standard ID | SN/T 4780-2017 (SN/T4780-2017) | Description (Translated English) | Detection of spoilage yeasts in food for export--Real-time PCR method | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | C53 | Word Count Estimation | 10,146 | Date of Issue | 2017-05-12 | Date of Implementation | 2017-12-01 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 228 | Issuing agency(ies) | General Administration of Customs |
SN/T 4780-2017: Detection of spoilage yeasts in food for export--Real-time PCR method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of spoilage yeasts in food for export--Real-time PCR method
China's entry-exit inspection and quarantine industry standards
Export food spoilage yeast
Real-time fluorescence PCR detection method
Published on.2017-05-12
2017-12-01 implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Inspection and Quarantine of the People's Republic of China
Industry Standard
Export food spoilage yeast
Real-time fluorescence PCR detection method
China Standard Press Publishing
First edition of February.2018
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard was drafted by. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and Shanghai Entry-Exit Inspection and Inspection
Epidemic Bureau.
The main drafters of this standard. Fu Zibo, Guo Weilei, Han Yu, Xu Hao, Rao Hong, Wang Chuanxian, Liu Xia, Li Xiaohong.
Export food spoilage yeast
Real-time fluorescence PCR detection method
1 Scope
This standard stipulates the Zygosaccharomyces rouxii and Saccharo-
Mycescerevisiae) and Brettanomycespp. Real-time fluorescence PCR detection.
This standard applies to the real-time fluorescence PCR detection of Ruthenium-binding yeast, Saccharomyces cerevisiae and Brewer's yeast in exported foods.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB 4789.15 National Food Safety Standard Food Microbiology Examination Mold and Yeast Count
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB 19489 General Requirements for Laboratory Biosafety
GB/T 27403 laboratory quality control standard food molecular biology test
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Spoilageyeasts
A spoilage yeast is a type of yeast that grows in food and causes food spoilage. Corrupt yeast breaks down sugar in food,
Colloids, starches and fats, produce carbon dioxide, sulfides, or cause food acidification, leading to food spoilage. Luw combines yeast, winemaking
Yeast and Brettanomyces are both important yeasts that cause food spoilage.
3.2
Luw combines yeast Zygosaccharomyces rouxi
The Rugby-binding yeast (Zygosaccharomyces rouxii) belongs to the family Sporocystomycetes, which is a member of the genus Ruthenium. Ruthenium binding yeast
Colonies on wort agar appear creamy, slightly shiny, soft and smooth in texture, oval or spherical.
Note. Luwkin's yeast binding is the most common hyperotrophic yeast. It is not only the producer of major traditional fermented foods, but also causes some low-water activity foods.
Spoilage bacteria of products and raw materials. Such as. Luk's yeast binding is an important strain producing fermented products such as soy sauce, Japanese miso and Thai fermented fish products. in
Juices, jams, soft drinks, salad dressings and ketchup are spoilage bacteria.
3.3
Saccharomyces cerevisiae
Saccharomyces cerevisiae belongs to the yeast family, Saccharomyces cerevisiae. Single cell, oval or spherical, with
Cell wall, cytoplasmic membrane, nucleus, liquefaction, mitochondria. The cell size is 2.5μm~10μm×4.5μm~21μm. Saccharomyces yeast
Smooth, moist, red, pink, or white wax-like colonies on the red medium of Bangladesh, often with stickiness. Saccharomyces cerevisiae
Budding yeast, often found in fruit and vegetable juice beverages, causes the beverage to spoil.
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