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Detection and identification of Dickeya chrysanthemi
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SN/T 4730-2016
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Basic data | Standard ID | SN/T 4730-2016 (SN/T4730-2016) | | Description (Translated English) | Detection and identification of Dickeya chrysanthemi | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 12,111 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs |
SN/T 4730-2016: Detection and identification of Dickeya chrysanthemi---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Dickeya chrysanthemi
People's Republic of China Entry - Exit Inspection and Quarantine Industry Standard
Quarantine identification method of chrysanthemum
2016-12-12 release
2017-07-01 implementation
People's Republic of China
State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and approved by the National Certification and Accreditation Administration Committee.
The drafting unit of this standard. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of the People's Republic of China
Bureau of the People's Republic of China Shenzhen Entry - Exit Inspection and Quarantine Bureau, the People' s Republic of China Fujian Entry - Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Yi Jianping, Jiao Binbin, Liao Furong, Feng Jianjun, Li Min, Yu Cui, India Liping.
Quarantine identification method of chrysanthemum
1 Scope
This standard specifies the quarantine identification method for Dickeyachrysanthemi.
This standard applies to the entry and exit of the ball, flowers and seedlings and other plant propagation materials in the chrysanthemum-based rot fungi quarantine identification.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB 4789.10-2010 Food Safety National Standard Food Microbiology Test Staphylococcus aureus Test
Code of quarantine for seedling of inbound and outbound plants
Sampling method for quarantine of inbound and outbound plants and plant products
Code for routine detection of plant pathogenic bacteria
3 Chrysanthemum Pyrule Basic information
Scientific name. Dickeyachrysanthemi (Burkholderetal.1953) Samsonetal.2005
Classification status. Proteobacteria, Gamma-proteobacteria, Enterobac-
teriales Enterobacteriaceae, Dickeya.
For additional information on chrysanthemum rot fungi, see Appendix A.
4 Principle of the method
In this standard, the symptoms, colony morphological characteristics, physiological and biochemical characteristics, Biolog test results, tobacco allergic reactions
As well as the pathogenicity test as the main basis for the development of chrysanthemum-based rot fungi quarantine identification method.
5 equipment and main reagents
5.1 Instruments and equipment
Clean bench, balance, Biolog bacteria identification system, low temperature refrigerator, light incubator, autoclave. Petri dishes, centrifuged
Tube, micro adjustable pipette, tip, scalpel, syringe and needle.
5.2 Main reagents
Beef extract, peptone, agar, yeast extract, sodium chloride, potassium chloride, dipotassium hydrogen phosphate, phenol red, hydrochloric acid arginine, glucose, bromophenol blue.
6 detection
6.1 symptom check
Samples were taken as required by SN/T 1157, SN/T 2601 and SN/T 2122 to check for symptoms of soft rot on the sample. Symptoms see attached
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