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SN/T 4730-2016 English PDF

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SN/T 4730-2016: Detection and identification of Dickeya chrysanthemi
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Basic data

Standard ID SN/T 4730-2016 (SN/T4730-2016)
Description (Translated English) Detection and identification of Dickeya chrysanthemi
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Word Count Estimation 12,111
Date of Issue 2016-12-12
Date of Implementation 2017-07-01
Regulation (derived from) State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies) General Administration of Customs

SN/T 4730-2016: Detection and identification of Dickeya chrysanthemi

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Detection and identification of Dickeya chrysanthemi People's Republic of China Entry - Exit Inspection and Quarantine Industry Standard Quarantine identification method of chrysanthemum 2016-12-12 release 2017-07-01 implementation People's Republic of China State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and approved by the National Certification and Accreditation Administration Committee. The drafting unit of this standard. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine of the People's Republic of China Bureau of the People's Republic of China Shenzhen Entry - Exit Inspection and Quarantine Bureau, the People' s Republic of China Fujian Entry - Exit Inspection and Quarantine Bureau. The main drafters of this standard. Yi Jianping, Jiao Binbin, Liao Furong, Feng Jianjun, Li Min, Yu Cui, India Liping. Quarantine identification method of chrysanthemum

1 Scope

This standard specifies the quarantine identification method for Dickeyachrysanthemi. This standard applies to the entry and exit of the ball, flowers and seedlings and other plant propagation materials in the chrysanthemum-based rot fungi quarantine identification.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB 4789.10-2010 Food Safety National Standard Food Microbiology Test Staphylococcus aureus Test Code of quarantine for seedling of inbound and outbound plants Sampling method for quarantine of inbound and outbound plants and plant products Code for routine detection of plant pathogenic bacteria

3 Chrysanthemum Pyrule Basic information

Scientific name. Dickeyachrysanthemi (Burkholderetal.1953) Samsonetal.2005 Classification status. Proteobacteria, Gamma-proteobacteria, Enterobac- teriales Enterobacteriaceae, Dickeya. For additional information on chrysanthemum rot fungi, see Appendix A.

4 Principle of the method

In this standard, the symptoms, colony morphological characteristics, physiological and biochemical characteristics, Biolog test results, tobacco allergic reactions As well as the pathogenicity test as the main basis for the development of chrysanthemum-based rot fungi quarantine identification method.

5 equipment and main reagents

5.1 Instruments and equipment Clean bench, balance, Biolog bacteria identification system, low temperature refrigerator, light incubator, autoclave. Petri dishes, centrifuged Tube, micro adjustable pipette, tip, scalpel, syringe and needle. 5.2 Main reagents Beef extract, peptone, agar, yeast extract, sodium chloride, potassium chloride, dipotassium hydrogen phosphate, phenol red, hydrochloric acid arginine, glucose, bromophenol blue.

6 detection

6.1 symptom check Samples were taken as required by SN/T 1157, SN/T 2601 and SN/T 2122 to check for symptoms of soft rot on the sample. Symptoms see attached