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SN/T 4723-2022 English PDF

SN/T 4723: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 4723-2022EnglishRFQ ASK 3 days [Need to translate] (Root knot nematode (Chinese species) quarantine and identification method) Valid SN/T 4723-2022
SN/T 4723-2016EnglishRFQ ASK 8 days [Need to translate] Detection and identification of Meloidogyne spp. (non-Chinese species) Obsolete SN/T 4723-2016

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Basic data

Standard ID SN/T 4723-2022 (SN/T4723-2022)
Description (Translated English) (Root knot nematode (Chinese species) quarantine and identification method)
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.20
Date of Issue 2022-07-07
Date of Implementation 2023-02-01
Older Standard (superseded by this standard) SN/T 4723-2016
Issuing agency(ies) General Administration of Customs

SN/T 4723-2016: Detection and identification of Meloidogyne spp. (non-Chinese species)

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(Method of quarantine identification of root - knot nematode (non - Chinese species)) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Quarantine identification method for root-knot nematode (non-Chinese species) Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Ningbo Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Tianjin Entry-Exit Inspection and Quarantine Bureau, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Nanjing Agricultural University, South China Agricultural University. The main drafters of this standard. Gu Jianfeng, Wang Jincheng, He Jie, Zhang Huili, Wang Wei, Li Fangrong, Lin Yu, Zhuo Yu. Quarantine identification method for root-knot nematode (non-Chinese species)

1 Scope

This standard specifies the quarantine and identification methods for root-knot nematode Meloidogyne species and non-Chinese species. This standard applies to the identification of root-knot nematodes in inland and outbound plants, plant products, soils and media.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 24829 Phytophthora genus (transfer type) quarantine and identification method Quarantine Regulations for SN/T 1157 Entry and Exit Plant Seedlings SN/T 2117 pseudo-Colombian root-knot nematode quarantine identification method 3 root-knot nematode basic information Scientific name. MeloidogyneGoeldi, 1887 English name. Root-knotnematodes Classification status. Tylenchida, Tylenchoidea, Hoppliumimidae, root branch subfamily (Meloidogyninae), Meloidogyne. Routes of transmission. Nematode root tissues, bulbs, bulbs and contaminated soil are the main carriers of root-knot nematode transmission. Long distance propagation Mainly by means of the transfer of infected plant propagation materials; the bonding of agricultural machinery and personnel in farming operations, as well as irrigation and wind and rain Causes a close spread. See Appendix A for additional information on root-knot nematodes. Nematodes of the root-knot nematode are obligate endoparasite nematodes of underground tissues such as plant roots, bulbs and bulbs, and their hosts and distribution range are very widely. At present, there are 98 known nematodes, the most common of which are M. incognita and P. elegans. M. arenaria, M. javanica, M. hapla, and M. ererolobii, I. The country has recorded nearly 40 species, 23 of which are identified as Chinese species (see Appendix B).

4 Principle of the method

The second instar larvae of the root-knot nematode are linear in the roots and roots. After invading the roots of the host, they are fixed and parasitized, and gradually develop into oval females. The worm causes the roots of the host to expand and become rooted. Therefore, if a suspected root knot is found, the female can be directly necropsy, or the host plant roots and Second-instar larvae or males are isolated from the soil or medium. After obtaining the nematode, it is discriminated according to the morphology of its female, male and second instar larvae. Whether it is a root-knot nematode, and then further use a combination of morphology and molecular biology, and refer to its host, geographical distribution and other information, Determine the type. A total of 98 root-knot nematodes have been reported, 40 of which have been reported in China. According to the literature, the frequency and distribution are wide. Whether the pan, the description is sufficient, etc., 23 of them are Chinese species, and the remaining 17 species are Chinese species to be further verified. If it is judged