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SN/T 4729-2016: Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones
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Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones
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SN/T 4729-2016
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Basic data | Standard ID | SN/T 4729-2016 (SN/T4729-2016) | | Description (Translated English) | Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,111 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4729-2016: Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones
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Detection and identification of Seifertia azaleae (Peck)Partridge and Morgan-Jones
China's entry-exit inspection and quarantine industry standards
The quarantine and identification method of aphid bud disease
Published on.2016-12-12
2017-07-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard was drafted by. China Academy of Inspection and Quarantine.
Drafters of this standard. Wu Pinshan, Du Hongzhong, Lei Rong, Shao Si, Yan Jin, and Zhang Qiuji.
The quarantine and identification method of aphid bud disease
1 Scope
This standard specifies the quarantine and identification methods for aphid blight germs.
This standard applies to aphid blight fungi carried in flower buds, leaf buds and shoots of Rhododendron L. plants.
Quarantine identification.
2 basic information of germs
Scientific name. Seifertiaazaleae (Peck) Partridge and Morgan-Jones
English name. Budblight, Budandtwigblight
Taxonomic status. Fungi, Ascomycota, Asperycota, Erythomycetes, Chaetothyri-
Ales, Herpetrichielaceae.
Route of transmission. The germs mainly remain on the disease buds or overwinter. Infested by the base of bud scales in the leap year and entered the pith, impeding water in the shoots
Convey the buds and shoots wither. Proximity is mainly caused by vector insects such as leafhoppers, bees, etc., and splashes of rain droplets. Long-distance transmission is mainly through the band
Diseased plants spread.
See Appendix A for additional information on aphid blight pathogens.
3 Methodology
The morphological characteristics and ITS gene sequence alignment characteristics of A. vegetative aphid were used as identification criteria.
4 Instruments and Equipment
4.1 Equipment and equipment
Biological microscope (with measurement function), stereo microscope, clean bench, biological incubator, balance, autoclave, desktop high-speed centrifugation
Machine, pH meter, water bath, PCR, electrophoresis, gel imager.
4.2 Appliances
Beakers, Erlenmeyer flasks, graduated cylinders, test tubes, petri dishes, alcohol lamps, tweezers, scissors, scalpels, inoculation needles, slides, coverslips, plastic mortars,
Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tanks.
5 Reagents and culture media
5.1 Reagents
Glucose, agar powder, sodium hypochlorite, streptomycin sulfate, liquid nitrogen, Tris-HCl, MgCl2, HCl, EDTA, NaOH, NaOAC,
KCl, Tris, CTAB, TAE, Ethanol, Phenol, EB, Chloroform, Isopropanol
(Isopropanol), Isoamylalcohol, Proteinase K, Taq DNA Polymerase, dNTPs, Primer ITS5/ITS4.
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