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SN/T 4656.8-2020 English PDF

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SN/T 4656.8-2020: (Import and export textile biosafety inspection methods - Part 8: General rules)
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Standard similar to SN/T 4656.8-2020

GB/T 32614   GB 31701   GB 18401   SN/T 4656.10   SN/T 4656.9   SN/T 4656.7   

Basic data

Standard ID SN/T 4656.8-2020 (SN/T4656.8-2020)
Description (Translated English) (Import and export textile biosafety inspection methods - Part 8: General rules)
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard W09
Classification of International Standard 59.080.99
Word Count Estimation 8,885
Date of Issue 2020-08-27
Date of Implementation 2021-03-01
Regulation (derived from) General Administration of Customs Announcement No. 98 [2020]
Issuing agency(ies) General Administration of Customs

SN/T 4656.8-2020: (Import and export textile biosafety inspection methods - Part 8: General rules)

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Biosafety testing methods of the textiles for import and export Part 8.General rules The People's Republic of China Entry-Exit Inspection and Quarantine Industry Standards 2020-08-27 release 2021-03-01 implementation Issued by the General Administration of Customs of the People's Republic of China

Foreword

SN/T 4656 "Methods for Biosafety Inspection of Imported and Exported Textiles" is divided into the following parts. --Part 1.Candida albicans; --Part 2.Escherichia coli; --Part 3.Coliform flora; --Part 4.Staphylococcus aureus; --Part 5.Total number of colonies; --Part 6.Salmonella; --Part 7.Pseudomonas aeruginosa; --Part 8.General Rules This part is part 8 of SN/T 4656. This part was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This standard is proposed and managed by the General Administration of Customs of the People's Republic of China. Drafting organizations of this section. Zhengzhou Customs of the People's Republic of China, Shijiazhuang Customs of the People's Republic of China. The main drafters of this section. Guo Huiqing, Li Ke, Zhang Shuxia, Yu Jianying, Lian Sumei, Xu Chao, Miao Li. Import and export textile biosafety inspection methods Part 8.General Rules

1 Scope

SN/T 4656 This part specifies the general basic principles and requirements for microbiological testing in import and export textiles. This section applies to microbiological inspections in import and export textiles.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 8170 Numerical rounding rules and the expression and determination of limit values GB 19489 Laboratory Biosafety General Requirements SN/T 1538.1 Guidelines for the Preparation of Culture Medium Part 1.General Rules for Quality Assurance of Laboratory Culture Medium Preparation SN/T 1538.2 Medium Preparation Guide Part 2.Practical Guide for Medium Performance Testing

3 Laboratory resource requirements

3.1 Personnel 3.1.1 The inspectors should have basic knowledge of textiles and professional theoretical knowledge of microorganisms. 3.1.2 The inspector shall have sufficient conditions to obtain continuous professional education and training experience, be able to understand the test procedures, perform the inspection correctly, Judge the result correctly and issue the test report correctly. 3.1.3 Inspectors should have sufficient knowledge of laboratory safety and have basic common sense of biological safety precautions, fire protection, and emergency response. 3.1.4 People with color vision impairment cannot perform experiments that require color discrimination, such as microscope observation and biochemical tests. 3.1.5 Inspectors should be familiar with laboratory quality control requirements, have certain practical experience, and be able to provide customers with necessary consultation and solutions Interpretation service. 3.2 Environment 3.2.1 The overall layout of the laboratory should adopt a unidirectional workflow to avoid cross-contamination. 3.2.2 The ambient temperature, humidity, cleanliness, illuminance and noise in the laboratory should meet the working requirements. 3.2.3 The inspection of general textile samples should be carried out in clean areas (including ultra-clean workbenches, biological safety cabinets, and sterile rooms). To effectively monitor and record environmental conditions. 3.2.4 The isolation and identification of pathogenic microorganisms must be carried out in a Class II biological safety cabinet. 3.2.5 When pathogenic microorganisms carry out nucleic acid amplification and detection, there should be four independent working areas (reagent preparation and storage area, nucleic acid extraction Area, nucleic acid amplification area, amplification product analysis area), and ensure that each area has the required special equipment. 3.2.6 The laboratory should be equipped with biosafety facilities and personal protective equipment that meet the biosafety requirements, such as biosafety cabinets and special hand washing Pools, eyewash devices, etc. 3.2.7 The different working areas of the laboratory should be clearly marked, and the biological hazard corresponding to the biological safety level of the testing activity should be used correctly. Harm identification. 3.3 Equipment 3.3.1 Commonly used equipment in microbiology laboratory includes. balance, drying oven, autoclave, pH meter, homogenizer, centrifuge, constant temperature culture Incubators, water baths, microscopes, refrigerators, freezers, biological safety cabinets, automatic microbial biochemical identification systems, etc. 3.3.2 The quantity and technical parameters of laboratory equipment should meet the needs of microbiological inspection work. 3.3.3 The experimental equipment should be placed under suitable environmental conditions, convenient for maintenance, cleaning, disinfection and calibration, and kept tidy and in good condition. Working status. 3.3.4 Laboratory equipment should be regularly inspected, calibrated and/or verified, maintained and maintained to ensure work performance and operational safety. 3.3.5 Key experimental equipment (such as autoclave, constant temperature incubator, automatic microbial biochemical identification system, etc.) must be used daily Record and monitor records regularly. 3.4 Inspection supplies 3.4.1 When testing textiles, conventional inspection supplies mainly include tweezers, scissors, stainless steel rulers or specification plates, beakers, measuring cylinders, and full filters. Net sterile homogenization bag, triangular flask, pipette tip, test tube, plate, inoculation loop, glass rod and L-shaped glass rod, etc. 3.4.2 The inspection supplies should be sterilized in an appropriate manner before use. The commonly used sterilization methods for inspection supplies are mainly moist heat (autoclave). Bacteria pot sterilization) and dry heat method (baking at high temperature in a drying oven) Both damp heat and dry heat sterilization are when items are exposed to high temperatures Kill the carried microorganisms, and strictly distinguish which sterilization method is suitable for the inspection supplies used, so as to avoid inspecting the supplies after high temperature. been destroyed. Metal products (such as scissors, etc.) and glass products (such as test tubes, etc.) in commonly used inspection supplies can be dried Heat sterilization, when other sterilization needs to be sterilized, moist heat can be used. During sterilization, the inspection supplies should be placed in a specific container or suitable for use. Wrap or stopper with suitable materials (such as special wrapping paper, aluminum foil paper, etc.). 3.4.3 Sterilized inspection supplies should be stored in a dry and clean environment, kept clean and/or sterile; they should be clearly marked; experimenters should Regularly check the sterilization effect of inspection supplies. 3.4.4 Disposable articles suitable for microbiological testing can be selected to replace reusable articles and materials (such as disposable petri dishes, suction Tubes, pipette tips, test tubes, inoculation loops, etc.). 3.4.5 During the inspection process, the unused inspection supplies cannot be used after opening the package; the inspection supplies must be properly carried out after use. Way of sterilization. 3.5 Media and reagents 3.5.1 The quality and preparation of culture media and reagents should meet the requirements of relevant project standards. 3.5.2 The preparation and quality assurance of the culture medium shall be prepared and tested in accordance with the requirements of SN/T 1538.1 and SN/T 1538.2.If used Commercially available dehydrated synthetic culture media should be selected from regular manufacturers, checked and accepted before use, and used according to their instructions. 3.5.3 The relevant information and records of the acceptance, preparation, and test performance of the culture medium and reagents should be kept. 3.5.4 The prepared culture medium and reagents should be clearly marked, and the content should include. name, preparation date, expiration date and preparer, etc. information. 3.5.5 Unless otherwise specified, all experimental reagents are analytically pure; the water used for nucleic acid detection experiments conforms to the first grade water in GB/T 6682 Requirements. 3.6 Quality control strains 3.6.1 The laboratory should store quality control strains that can meet the needs of the work. 3.6.2 Laboratory quality control strains are generally used for the evaluation of testing methods, the verification and calibration of instruments and equipment, the verification of testing proficiency, culture media and Laboratory quality activities such as quality control of testing reagents, testing process and result evaluation. 3.6.3 Laboratory quality control strains generally come from professional authorities or accreditation departments, and can also be obtained from other traceable sources. 3.6.4 For the original isolated strains that are separated, purified, and identified in daily testing, and are not registered with the specialized agency for microbial strain preservation (Wild strains) Perform a systematic and complete record of strain information, including isolation time, source, main morphological characteristics, and final identification Make conclusions and so on. After preservation, it can be used as a reference for quality control strains. 3.6.5 In the process of using quality control strains, the laboratory shall ensure the biological characteristics and purity of the quality control strains, and receive, pass down, identify, and identify the quality control strains. Standardized management of preservation, destruction and records. 3.6.6 The laboratory's use, identification, passaging, and destruction of quality control strains should meet the biosafety requirements.

4 General principles of sample collection

4.1 Principles of sample collection 4.1.1 The principle of representativeness Determine the sampling plan according to the inspection purpose, textile characteristics, batch size, inspection method, microbial hazard degree, etc., random sampling, The collected samples can truly reflect the overall level of the sampled. 4.1.2 The principle of typicality Collect typical samples that can fully demonstrate the purpose of monitoring. 4.1.3 The principle of appropriateness The number of samples collected should meet the requirements of inspection and sample retention, and should not cause waste. 4.1.4 The principle of sterility Sampling should meet the requirements of aseptic operation. Sampling tools and sampling bags should be sterile, dry, leak-proof, suitable in shape and size, and one The sampling bag can only hold one sample to prevent cross-contamination. 4.1.5 Timeliness principle All sample collection should be completed in the shortest possible time, and samples should be sent for inspection as soon as possible after collection. During storage and transportation, The environment should be as consistent as possible with the original storage conditions of the samples to prevent changes in the number of microorganisms. 4.1.6 Principles of Procedure Sampling, delivery for inspection, retention of samples and issuance of reports are all carried out in accordance with the prescribed procedures. All stages should have complete procedures and clear handover. 4.1.7 Same principle When collecting samples, the test and reserve samples should be the same sample, that is, the same manufacturer, the same brand, the same specification, the same production date, Same batch number. 4.2 Sample collection 4.2.1 Determine the sampling plan according to the inspection purpose and product requirements, and prepare the sampling utensils. 4.2.2 Samples of the same batch, individually packaged, and appropriate number of pieces should be collected and sent for inspection. 4.2.3 The collection of textile samples in biosafety incidents, the biosafety incidents caused by the contamination of industrialized mass production and processing of textiles The collection of samples is carried out in accordance with 4.2.1 and 4.2.2.At the same time, ensure that there are textile samples remaining on the collection site. By the textile public The use of textiles (such as hospitals, hotels, etc.) or households in the use of biological safety incidents caused by the use of textiles, textiles The collection of fabric samples is based on 10% of the total put into use. When the total put into use of a certain type of textile is less than 10 pieces, this The sampling quantity of such items should be at least 1 piece. To meet the requirements of pathogen determination and pathogen confirmation of biosecurity incidents. 4.2.4 For the marking of samples, the collected samples shall be recorded and marked timely and accurately. The sampler shall clearly fill in the sampling sheet (including Including sampling person, sampling location, time, sample name, source, batch number, quantity, storage conditions and other information).

5 General requirements for inspection

5.1 Sample processing 5.1.1 After receiving the sample, the laboratory should first check the sample status, package integrity, source, storage information and record. 5.1.2 Record the inspection requirements and inspection items, and carry out the inspection as soon as possible as required. 5.1.3 Before opening the sample bag, wipe its surface clean, and then disinfect the opening part and its surroundings with 75% alcohol to ensure aseptic operation. 5.2 Sample preparation method 5.2.1 Weighing method Accurately weigh 25 g of sample. After cutting, add to 225 mL sterile diluent, beat with a flapping homogenizer for 1 min to 2 min, Mix well to get a 1.10 sample solution. 5.2.2 Multi-point sampling elution method 5 sampling points are evenly distributed around and in the middle of the sample, using a sterile specification plate, and each sampling point is 4 cm×5 cm (20 cm2) surface area. The product range is tailored, and the sampling area is 1 inspection sample for every 20 cm2.A total of 5 inspection samples are collected for each textile, and the sampling area is 100 cm2.will The 5 samples collected above are put into a sterile homogenization bag containing.200 mL of sterile diluent with a full filter mesh, and beat with a flapping homogenizer for 1 min ~ 2 min, mix thoroughly to obtain a sterile eluent, and prepare the sample solution as the original solution. 5.2.3 Cotton swab smearing method Moisten sterile and dry cotton swabs with sterile diluent, distribute 5 sampling points evenly around and in the middle of the sample, and use 1 for each sampling point. A sterile cotton swab, encircle the volume with a sterile specification board, smear it evenly in the area of 4 cm×5 cm (20 cm2), and immediately cut it with sterile scissors Remove the hand contact part of the cotton swab, put the smeared part into a 50 mL sterile diluent to make a sample uniform solution of 1.10. 5.2.4 Selection of sample preparation method 5.2.4.1 The sample preparation is generally based on the method 5.2.1.25 g sample 225 mL sterile diluent is the standard method. 5.2.4.2 When the sample is larger or thicker or porous, the sample preparation method should be used in 5.2.2.When using 5.2.2 method, if If the sample area is too large or too small, the sampling points can be increased or decreased proportionally. 5.2.4.3 When the sample is dense and not easy to be cut and prepared, or the sample is expensive, and the customer requires non-destructive testing, the sample preparation should be used 5.2.3 Method. 5.2.4.4 When methods 5.2.2 and 5.2.3 are used, if the tested sample absorbs a large amount of water, which results in insufficient sample homogenization and transfer, the sterile The diluent can be increased appropriately until enough to be sucked out for conversion. 5.3 Inspection method 5.3.1 Priority should be given to the currently effective standard methods. 5.3.2 Prior to the introduction of standard methods for testing, the laboratory should use natural contaminated samples...

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