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SN/T 4290-2015 English PDF

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SN/T 4290-2015: Quarantine protocol for Ichthyophthirius multifiliis
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Basic data

Standard ID SN/T 4290-2015 (SN/T4290-2015)
Description (Translated English) Quarantine protocol for Ichthyophthirius multifiliis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Word Count Estimation 12,175
Date of Issue 2015-09-02
Date of Implementation 2016-04-01
Quoted Standard GB/T 6682; GB/T 19495.2
Regulation (derived from) AQSIQ Announcement 2015 - Batch 3 - 103 Industry Standards
Issuing agency(ies) General Administration of Customs
Summary This standard specifies more children Ichthyophthirius clinical diagnosis, morphological diagnosis and PCR detection method. This standard applies to many children Ichthyophthiriasis pathogen identification and epidemiological investigation and monitoring.

SN/T 4290-2015: Quarantine protocol for Ichthyophthirius multifiliis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Ichthyophthirius insect quarantine technical specifications) People's Republic of China Entry-Exit Inspection and Quarantine Standards More children Ichthyophthirius quarantine Technical Specifications Issued on. 2015-09-02 2016-04-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine Bureau Technology Center, People's Republic of China Hainan Exit Inspection and Quarantine. The main drafters of this standard. Guo Shulin, Chen Xinzhong, Wushan Nan, Gong Yanqing, Yang Junping, Huang Ji Hui, Fan Germany. More children Ichthyophthirius quarantine Technical Specifications

1 Scope

This standard specifies more children Ichthyophthirius clinical diagnosis, morphological diagnosis and PCR detection method. This standard applies to many children Ichthyophthiriasis pathogen identification and epidemiological investigation and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.2 technical requirements for GMO testing laboratories

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Trophozoites trophont Adults adult Ichthyophthirius parasitic worm stage, referring to the infective larvae enter the skin or gills of fish, the fish to mature and leave the stage. 3.2 Cysts tomont Parasites on the host trophozoites from the host in water freely formation of cysts. 3.3 Larvae theront The formation of large cysts successive divisions larvae break out of the capsule, forming infective larvae grow.

4 Disease Overview

Disease caused by Ichthyophthirius insects (Ichthyophthiriusmultifiliis) caused by parasitic fish called multiple sub Ichthyophthiriasis (ich- thyophthiriasis), also known as white spot disease. Pest for most freshwater and brackish water fish are susceptible. Fish because the pest infection, and because of the Injury secondary to other pathogenic infections caused by worms, can cause large numbers of deaths. More children Ichthyophthiriasis details, see Appendix A.

5 Reagents and materials

5.1 All chemicals were of analytical grade. Test water should comply with GB/T 6682 in a water specifications. For DNA extraction and PCR experiments need to use water sterilized double distilled water. 5.2 Shaoding Shi fixative (Schaudinn'ssolution) (preparation see Appendix B B.1).

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