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SN/T 3747-2013: Detection and identification of Ovulinia azaleae Weiss
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3747-2013 | English | 319 |
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Detection and identification of Ovulinia azaleae Weiss
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SN/T 3747-2013
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Basic data | Standard ID | SN/T 3747-2013 (SN/T3747-2013) | | Description (Translated English) | Detection and identification of Ovulinia azaleae Weiss | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,169 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Magpies took Du Du magpie on host plants and flowers Fusarium quarantine and identification methods. This standard applies to Mrs Magpie flowers wilt quarantine and identification of bacteria. | SN/T 3747-2013: Detection and identification of Ovulinia azaleae Weiss---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Ovulinia azaleae Weiss
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Azalea quarantine and identification of Fusarium oxysporum
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Ningbo Exit Inspection and Quarantine.
The main drafters of this standard. the military section of Victoria, Guo Lixin, Chen Xianfeng, Zhang Huili.
Azalea quarantine and identification of Fusarium oxysporum
1 Scope
This standard specifies the azaleas on host plants like azaleas Fusarium quarantine and identification methods.
This standard applies to azalea wilt quarantine and identification of bacteria.
2 azalea Fusarium oxysporum basic information
Scientific name. OvuliniaazaleaeWeiss, 1940
Formerly known as. Sclerotiniaazaleae (FAWeiss) Dennis, (1956)
Anamorph. OvulitusazaleaeBuchwald
Taxonomic position. Fungi (Fungi), Ascomycotina (Ascomycota), Ascomycetes (Ascomycetes), flexible membrane bacteria mesh (Helo-
tiales), nuclear pezizaceae (Sclerotiniaceae), Beauveria Sclerotinia spp (Ovulinia).
Transmission. the bacteria can be wind, rain, insects and field operations spread Essay disease petals over long distances.
Other information contained in the bacteria in Appendix A.
3 PRINCIPLE OF THE METHOD
In azaleas wilt pathogen culture traits and morphological characteristics of the pathogen as a basis for identification.
4 Equipment and Reagents
4.1 Equipment
Microscope (with measurements), stereo microscope, clean benches, biological incubator, scales, autoclaves, beakers, flasks,
Cylinder, test tubes, Petri dishes (diameter 9cm), alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, fluorescent lamps, filter paper.
4.2 Reagents
Sodium hypochlorite, corn flour, agar powder, anhydrous ethanol.
4.3 Medium
Corn meal agar (CMA). Weigh corn flour 200g, add appropriate amount of distilled water and boil for about 20min, double gauze filtration,
Filtered juice was added agar 20g, continue to cook until the agar is completely dissolved, add distilled water to make up 1000mL, 121 ℃ autoclave 20min.
Identify bacteria 5
5.1 Symptom Check
Picked diseased plants were observed. Disease symptoms described in Appendix A.
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