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Detection and identification of Fusarium oxysporum f.sp.lilii
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SN/T 3746-2013
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Basic data | Standard ID | SN/T 3746-2013 (SN/T3746-2013) | | Description (Translated English) | Detection and identification of Fusarium oxysporum f.sp.lilii | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 13,163 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method to identify and quarantine Lily Fusarium. This standard applies to lily bulb scales carried withered lily quarantine and identification of bacteria. |
SN/T 3746-2013: Detection and identification of Fusarium oxysporum f.sp.lilii---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Fusarium oxysporum f.sp.lilii
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Lily quarantine and identification of Fusarium oxysporum
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Academy of Agricultural Sciences, Xiamen People's Republic of China
Door CIQ.
The main drafters of this standard. Qi Longjun, Songshao Yi, Dai Fuming, Zeng Rong, Yang Cuiyun, Wang Hongyi.
Lily quarantine and identification of Fusarium oxysporum
1 Scope
This standard specifies the method for inspection and identification Lily Fusarium.
This standard applies to lily bulb scales carried withered lily quarantine and identification of bacteria.
2 Basic information of germs
Chinese name. Lily Fusarium oxysporum
English name. lilyfusariumwilt
Name. Fusariumoxysporumf.sp.liliiSnyd.
Taxonomic position. asexual stage belongs. Fungi (KingdomFungi), imperfect fungi Amon (Deuteromycotina), Trichosporon Gang (Hy-
phomycetes), tumor seat spore head (Tuberculariales), tumor seat spore Branch (Tuberculariaceae), Fusarium (Fusarium). No
Sexual stage.
Transmission. There are. a) soil-borne, the spread of contaminated soil is one of the main ways of the spread of the pathogen; b) bulbs borne diseases, with
Bulbs disease spread disease through sale, transportation and other means; c) residues contaminated, sick balls debris large number of pathogens in the soil can be long-term
Survival, in the process of re-planting, can spread germs.
Lily Fusarium additional information, see Appendix A.
3 PRINCIPLE OF THE METHOD
This standard is based on the characteristic symptoms of harmful bacteria on the main lily bulb ball, morphological characteristics and PCR reaction characteristics identified one hundred
Together Fusarium basis.
4 apparatus and appliances
4.1 The main instruments and appliances
Clean bench, incubator, biological microscopes, stereo microscope organism, PCR instrument, gel electrophoresis, desktop refrigerated centrifuge, water
Bath incubator, ice maker, Vortex, electrophoresis, constant temperature water bath, UV transmission analyzer, gel imaging system, a mortar and pestle, the balance (flu
The amount of 0.0001g), white disk and pointed tweezers, pH meter, plastic bags, tubes and the like.
4.2 Reagents and culture medium
Sodium hypochlorite, Tris-HCl, sodium chloride, EDTA, chloroform, isoamyl alcohol, four kinds of deoxynucleotide triphosphates (dNTPs), ethidium bromide
(EB), RNase enzyme, polyvinylpyrrolidone (PVP), isopropanol, mercaptoethanol, bromophenol blue, CTAB, Taq enzyme. PSA medium
(Potato 200g, sugar 20g, Agar 20g, Distilled water 1000mL).
5 Identification of the Pathogen
5.1 Symptom Check
According to the characteristics of specific symptoms lily blight in lily bulb scales, well-lit place, for samples taken by one tuber inspection table
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