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Detection and identification of Sphaeropsis tumefaciens Hedges
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SN/T 3748-2013
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Basic data | Standard ID | SN/T 3748-2013 (SN/T3748-2013) | | Description (Translated English) | Detection and identification of Sphaeropsis tumefaciens Hedges | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,128 | | Quoted Standard | SN/T 2455 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the identification of phytosanitary quarantine of citrus branches tumor germs. This standard applies to the entry and exit inspection and identification of citrus and citrus branches in other tumor host bacteria. |
SN/T 3748-2013: Detection and identification of Sphaeropsis tumefaciens Hedges---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Sphaeropsis tumefaciens Hedges
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Branch citrus quarantine and identification of tumor germs
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry-Exit-Entry Inspection and Hubei People's Republic of China
Quarantine Bureau.
The main drafters of this standard. Wang Weifang, Zhao Lirong, gorgeous, Feng Lixia, Fed.
Branch citrus quarantine and identification of tumor germs
1 Scope
This standard specifies the method for identification of quarantine phytosanitary Citrus branch tumor germs.
This standard applies to the entry and exit inspection and identification of citrus and citrus branches in other tumor host bacteria.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2455 entry and exit inspection and quarantine procedures fruits
3 quarantine and identification principles
According citrus branch tumor germs Damage symptoms on the host, the bacteria culture traits and morphological characteristics, as identified by citrus branch tumor germs
according to. Taxonomic status citrus branch tumor germs, distribution, damage symptoms and host range and other background information, see Appendix A.
4 instruments and appliances
4.1 Instrument
Stereo microscope, biological microscope, clean benches, autoclaves, bio-incubator, pH meter.
4.2 Appliances
Sample bags, labels, magnifying glass, forceps, scalpels, alcohol lamp, inoculation needles, petri dish (diameter 9cm), beakers, flasks, test tubes, the amount of
Cylinder, slides, cover slips, hole punch (diameter 6mm).
5 Reagents and culture medium
95% ethanol, sterile water, potato dextrose agar medium (the PDA) (per 200g 1000mLPDA the peeled potatoes, 20g
Glucose, Agar 18g preparation), acidic potato dextrose agar medium (APDA) (4.0mL was added per 1000mLPDA
25% lactic acid).
Quarantine and identification of 6
6.1 port site inspection
6.1.1 Sampling
When port-site inspection, according to SN/T 2455 sampling and sampling.
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