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SN/T 3691-2013: PCR-DHPLC method for detection of genetically modified corn
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PCR-DHPLC method for detection of genetically modified corn
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Standard similar to SN/T 3691-2013 SN/T 5268 | Basic data | Standard ID | SN/T 3691-2013 (SN/T3691-2013) | | Description (Translated English) | PCR-DHPLC method for detection of genetically modified corn | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 10,183 | | Quoted Standard | GB/T 6682; GB/T 19495.2; GB/T 19495.7; SN/T 1193 | | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the PCR-DHPLC detection of transgenic corn product ingredients. This standard applies to products of transgenic maize line 59122, Btll, Bt176, CBH351, EV3272, GA21, MIR604, MON810, MON863, MON88017, MON89034, NK603, T25, and TC1507 | SN/T 3691-2013: PCR-DHPLC method for detection of genetically modified corn---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
PCR-DHPLC method for detection of genetically modified corn
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Transgenic maize PCR-DHPLC Detection
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection
Quarantine Science Research Institute, National Center for Nanoscience, Shenzhen Bo Rui Xiang Hui Biotechnology Co., Ltd., Liaoning Academy of Agricultural Sciences.
The main drafters of this standard. apricot Ling, Zhang Guiming, water Zhu Fang, Pan Guangxue, only to Yu, Cheng Yinghui, Longhai, Huang Xin, Wang Zhaohui, PLAIN
Ouyang Hui, Miao Jian Kun.
Transgenic maize PCR-DHPLC Detection
1 Scope
This standard specifies the PCR-DHPLC detection of transgenic maize product ingredients.
This standard applies to products transgenic corn line 59122, Bt11, Bt176, CBH351, EV3272, GA21, MIR604,
MON810, MON863, MON88017, MON89034, NK603, T25 and TC1507 detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 19495.2 technical requirements for GMO testing laboratories
GB/T 19495.7 GMO detection methods of sampling and sample preparation
SN/T 1193 genetic testing laboratory technical requirements
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
Denaturing high-performance liquid chromatography (DenaturingHigh-performanceLiquidChromatography, DHPLC)
An application of the principle of ion-pair reversed-phase liquid chromatography separation of DNA fragments is simple, fast, non-gel method for nucleic acid analysis.
3.2 Acronyms
The following abbreviations apply to this document.
BAR. phosphate tricine acetyltransferase gene (phosphinothricinacetyltransferasegene)
bp. base pairs (basepair)
CaMV35S. cauliflower mosaic virus 35S promoter (35Spromoterfromcauliflowermosaicvirus)
Ct value. number of cycles in each reaction tube fluorescent signal reaches the set threshold experienced (C. Cycle, t. threshold)
CTAB. cetyl trimethyl ammonium bromide (cetyltrithylammoniumbromide)
dATP. triphosphate (deoxyadenosinetriphosphate)
dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate)
dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate)
DNA. deoxyribonucleic acid (deoxyribonucleicacid)
dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate)
dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate)
dUTP. deoxyuridine triphosphate (deoxyuridinetriphosphate)
EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraaceticacid)
FMV35S. figwort mosaic virus 35S promoter (35SpromoterfromFigwortMosaicVirus)
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