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SN/T 3675-2013: Detection and identification of Rhizoctonia fragariae Husain et W.E.McKeen
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Detection and identification of Rhizoctonia fragariae Husain et W.E.McKeen
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Standard similar to SN/T 3675-2013 SN/T 5268 | Basic data | Standard ID | SN/T 3675-2013 (SN/T3675-2013) | | Description (Translated English) | Detection and identification of Rhizoctonia fragariae Husain et W.E.McKeen | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 13,181 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the strawberry blossom blight quarantine and identification methods. This standard applies to the strawberry seeds, seedlings and flowers withered strawberry fruit quarantine and identification of bacteria. | SN/T 3675-2013: Detection and identification of Rhizoctonia fragariae Husain et W.E.McKeen---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Rhizoctonia fragariae Husain et WEMcKeen
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Strawberry flower blight pathogen identification of quarantine
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China
State and Jiangsu Entry-Exit Inspection and Quarantine Xiamen People's Republic of China, People's Republic of China Shandong Entry-Exit Inspection
Quarantine Bureau, Tianjin, People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Wang Zhenhua, Pinghan Li, Zhang Jianfeng, Li Feng new, Wang Hongyi, leaf Yun Wu Shan goods, Wu Cuiping, Li Bin, Wang Premiership,
Roca Phoenix.
Strawberry flower blight pathogen identification of quarantine
1 Scope
This standard specifies the strawberry flower blight pathogen quarantine and identification methods.
This standard applies to the strawberry seeds, seedlings and fruits of strawberry flower blight quarantine and identification of bacteria.
2 Normative references
The following documents for the application of this document is essential For dated references, only the dated edition applies to this article
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Basic information of germs
Chinese name. strawberry flower blight pathogen
Name. RhizoctoniafragariaeHusainetW.E.Mckeen
Taxonomic position. strawberry flower blight pathogen anamorph Rhizoctoniafragariae belong Fungi Fungi, Deuteromycetes door
Basidiomycota, Trichosporon Gang Hyphomycetes, non-spore head Agonomycetales, non-spore Branch Agonomycetaceae, Rhizoctonia
Rhizoctonia. Sexuality Ceratobasidiumcornigerum, belonging burden Amon Basidiomycota, Agaricales Gang Agaricomyce-
tes, chanterelles head Cantharelales, Basidiomycetes angle Branch Ceratobasidiaceae, Tam Kok genus Ceratobasidium.
Transmission. contaminated strawberries, root, bud, Soil is the main way to spread long distances.
For additional information, see Appendix A.
4 principle of the method
According strawberry flower blight pathogen in the host Damage symptoms, morphological characteristics, cultural characteristics and other characteristics of the results of determination.
5 Equipment and Reagents
5.1 Equipment
PCR instrument, clean benches, autoclaves, ice maker, nucleic acid protein analyzer, high-speed refrigerated centrifuge, small desktop centrifuge, ultra
Cryogenic refrigerators, conventional refrigerator, Vortex, constant temperature water bath, micro-injector, electrophoresis, gel electrophoresis equipment, gel imaging system, stereo
Microscope, biological microscope (with a microscopic camera device), the balance (sense of volume 0.1g), micrometer, light incubator like.
5.2 Reagents and culture medium
Agarose powder, sodium hypochlorite (NaClO4), chloramphenicol, primers, Tris (Tris), cetyltrimethylammonium bromide
(CTAB), ethylene diamine tetra-acetic acid (EDTA), ethanol, proteinase K, phenol, chloroform, isoamyl alcohol, ammonium acetate (NH4Ac), chloride
Magnesium (MgCl2), PCR buffer, dNTPs (dATP, dTTP, dCTP, dGTP), TaqDNA polymerase, ethidium bromide (the EB), liquid
N, 3.5% formaldehyde, diamino-2-phenylindole (the DAPI), potassium nitrate (of KNO3), water and potato dextrose agar medium (PDA). real
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