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SN/T 3637-2013: Determination of isomaltose and tagatose in food for export. HPLC method
Status: Valid
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Determination of isomaltose and tagatose in food for export. HPLC method
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SN/T 3637-2013
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Basic data | Standard ID | SN/T 3637-2013 (SN/T3637-2013) | | Description (Translated English) | Determination of isomaltose and tagatose in food for export. HPLC method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 16,166 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the maltose, tagatose side HPLC method given different foods. This standard applies to fruit drinks, milk drinks, bees, candy, crackers, rice, bacon isomalt, tagatose side with children given. | SN/T 3637-2013: Determination of isomaltose and tagatose in food for export. HPLC method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of isomaltose and tagatose in food for export. HPLC method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of maltose, tagatose food exports iso
High Performance Liquid Chromatography
HPLCmethod
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hebei Exit Inspection and Quarantine.
The main drafters of this standard. Mayu Song, Doucai Yun, Guo Chunhai, Yilian Feng, Gu Haitao, Chenrui Chun, Duan Wenzhong.
Determination of maltose, tagatose food exports iso
High Performance Liquid Chromatography
1 Scope
This standard specifies a high performance liquid chromatographic method maltose, tagatose different food for export.
This standard applies to fruit drinks, measured maltose, tagatose content of milk drinks, honey, candy, cookies, cereal, bacon vary.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
Sample isomalt, tagatose water extraction, and the extract after protein precipitation with C18 column purification or directly to a C18 column purification, high performance liquid
Phase chromatography, external standard.
4 Reagents and materials
Unless otherwise indicated, reagents were of analytical grade water as a water GB/T 6682 regulations.
4.1 acetonitrile chromatography.
4.2 zinc acetate.
4.3 potassium ferrocyanide.
4.4 zinc acetate solution (219g/L). Weigh 219g zinc acetate (4.2), dissolved in water and diluted to 1L.
4.5 potassium ferrocyanide solution (106g/L). Weigh 106g of potassium ferrocyanide (4.3), dissolved in water and diluted to 1L.
4.6 Standard substance. isomalt (formula C12H22O11, CAS number. 499-40-1), Tagatose (molecular formula C6H12O6, CAS number.
87-81-0) purity of greater than or equal to 95%.
4.7 standard stock solution. accurately weighed amount of isomaltulose, tagatose standard substance (4.6), formulated as a water 50.0mg/mL of standard, respectively
Stock solution at 4 ℃ refrigerator.
4.8 Standard curve series. standard stock solution (4.7) with water progressively diluted isomalt concentration of 1.0mg/mL, 2.0mg/mL,
5.0mg/mL, 10.0mg/mL, 20.0mg/mL, the concentration of tagatose were 0.4mg/mL, 0.8mg/mL, 2.0mg/mL,
4.0mg/mL, the standard curve solution 8.0mg/mL, and prepared before use.
4.9 C18 SPE column, 500mg, 3mL. Before using successively with 5mL methanol and 5mL of water conditions, keeping the cylinder wetting.
4.10 0.45μm membrane, water.
5 instruments and equipment
5.1 High performance liquid chromatograph. equipped with a differential refractive index detector.
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