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SN/T 3625-2013: Determination of asarone in food for export. Liquid chromatographic method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3625-2013 | English | 469 |
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Determination of asarone in food for export. Liquid chromatographic method
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SN/T 3625-2013
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Basic data | Standard ID | SN/T 3625-2013 (SN/T3625-2013) | | Description (Translated English) | Determination of asarone in food for export. Liquid chromatographic method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 18,17 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of liquid chromatography side given the main ingredient in foods ��-asarone and ��-asarone content of white iris oil. This standard applies to vodka, liquor, pork, smoked fish measured, mung bean cake, seasoning powder, mi | SN/T 3625-2013: Determination of asarone in food for export. Liquid chromatographic method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of asarone in food for export. Liquid chromatographic method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of food for export Asarone Content
Liquid chromatography
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Peng Fang, Leimei Kang, Yang orchids, Xu Fei, Ding Huiying, Han Chao, Xu construction.
Determination of food for export Asarone Content
Liquid chromatography
1 Scope
This standard specifies the method of liquid chromatography in food the main component of asarone α- and β- asarone content of white iris oil.
This standard applies to vodka, liquor, pork, smoked fish, green bean cake, seasoning powder, milk powder and other food exports in α- and β- asarone Asarone
Determination of.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
The sample asarone extracted with acetonitrile, concentrated and purified by silica SPE column eluate was concentrated dissolved in methanol to volume, liquid
Phase chromatography with fluorescence detector, external standard. Alcoholic beverage filtration membrane sample was measured directly on the machine.
4 Reagents and materials
Water as a water GB/T 6682 regulations.
Ethyl acetate 4.1. HPLC grade.
4.2 acetonitrile. HPLC grade.
4.3 hexane. HPLC grade.
4.4 Methanol. HPLC grade.
4.5 Asarone standards. α- asarone. CAS No. 2883-98-9, relative molecular mass of 208.25, purity of greater than or equal to 98%;
β- asarone, CAS No. 5273-86-9, relative molecular mass of 208.25, purity of greater than or equal to 95%.
4.6 preparation of standard stock solution. accurately weighed amount of asarone standards, prepared in methanol at a concentration of 1.0mg/mL of standard stock
Preparation of the solution. The solution was formulated in a brown flask below -18 ℃ stored.
Preparation 4.7 standard intermediate solution. methanol concentration of the standard solution stepwise diluted to 1.0μg/mL. The solution was formulated in a brown container
Flask below -18 ℃ stored.
Preparation 4.8 standard working solution. If necessary, draw a certain amount of standard intermediate solution before use, the appropriate concentration prepared in methanol mixtures
Combined standard working solution. At 0 ℃ ~ 4 ℃ dark refrigerated.
4.9 silica SPE column. 1g, 6mL. Before using successively with 5mL of ethyl acetate, 5mL n-hexane activation.
4.10 microporous membrane. 0.45μm, organic.
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