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SN/T 3569-2013 PDF English

Std ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status
SN/T 3569-2013	English	319	Add to Cart	3 days [Need to translate]	Methods for the preservation of phytoplasma strain culture	Obsolete

Standard similar to SN/T 3569-2013

SN/T 5268 |

Basic data

Standard ID	SN/T 3569-2013 (SN/T3569-2013)
Description (Translated English)	Methods for the preservation of phytoplasma strain culture
Sector / Industry	Commodity Inspection Standard (Recommended)
Word Count Estimation	8,825
Quoted Standard	SN/T 2010-2007
Regulation (derived from)	?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165)
Issuing agency(ies)	General Administration of Customs
Summary	This standard specifies the phytoplasma strains in vivo preservation methods. This standard applies to save phytoplasma strains in vivo.

SN/T 3569-2013: Methods for the preservation of phytoplasma strain culture

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Methods for the preservation of phytoplasma strain culture People's Republic of China Entry-Exit Inspection and Quarantine Standards Phytoplasma strains in vivo preservation methods Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, Chinese Academy of Forestry. The main drafters of this standard. Zhao Wenjun, Mu Haiqing, Tian Guozhong, Songzhuan Sheng, Lin Caili, water Zhu Fang, China Guo Wei. Phytoplasma strains in vivo preservation methods

1 Scope

This standard specifies the phytoplasma strains in vivo conservation methods. This standard applies to save phytoplasma strains in vivo.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2010-2007 phytoplasma Removal Principle 3 3.1 phytoplasma turn parasite in the phloem of plants, no cell wall, is the only three-layer film bag, can not be cultured in vitro. In the absence of cell Wall, it is vulnerable to external environmental conditions, such as plants absorb water, osmotic concentration of cytosolic and intercellular, it presents more Formability. 3.2 phytoplasma mainly by feeding on plant phloem sap leafhoppers, aphids, planthoppers and other insect vectors spread, JWB phytoplasma spread main China intends to pass a ribbed leafhopper (HishimonuschinensisAnufriev), recessed edge Aya leafhopper (HishimonussellatusUhler), Orange with leafhoppers (HishimonusaurifacialeaKuah) and red flashing Xiaochan (Typhlocypaspp.) Transmission. 3.3 phytoplasma can also be spread by the media --- dodder plant. Dodder is a parasitic plant, by establishing a common transporting host System, drawing nutrients from the host plant, while phytoplasma can also spread to healthy plants from diseased plants by transporting system. So far, There has not been proved phytoplasma can not propagate by seed and mechanical methods like virus. Effective way phytoplasma artificial propagation is married Connection. 3.4 This standard based on the biological characteristics of phytoplasma and virus transmission characteristics. Related Information See Appendix A. 4 equipment, utensils and reagents 4.1 Equipment Electronic balance (inductance 1/10000g), artificial climate chamber, autoclave, clean benches, fluorescence microscopy, high-speed refrigerated centrifuge, Low-speed centrifuge, heated water bath, low temperature refrigerators, refrigerating, PCR instrument, electrophoresis, electrophoresis tank gel image analyzer, electric mixer Machine, tissue culture and inter-culture inoculation room. 4.2 Appliances Tubes, tissue culture flasks, scissors, tweezers, alcohol lamp, high-temperature autoclaves, pipette and tip, mortar, centrifuge tubes, PCR tubes, graduated cylinder, Beakers, pots, insect nets cover, budding knife. 4.3 Reagents 70% alcohol, 0.1% mercuric chloride.