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SN/T 3557-2013: RT-PCR and real time RT-PCR detection methods of yellow fever virus
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RT-PCR and real time RT-PCR detection methods of yellow fever virus
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Standard similar to SN/T 3557-2013 SN/T 5268 | Basic data | Standard ID | SN/T 3557-2013 (SN/T3557-2013) | | Description (Translated English) | RT-PCR and real time RT-PCR detection methods of yellow fever virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 10,194 | | Quoted Standard | GB 19489; human infectious pathogens directory (Ministry of Health) | | Regulation (derived from) | ?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the safety requirements for border crossings yellow fever biological detection, specimen collection, transport and storage, handling specimens, flavivirus conventional RT-PCR, RT-PCR and real-time fluorescence detection method. Thi | SN/T 3557-2013: RT-PCR and real time RT-PCR detection methods of yellow fever virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
RT-PCR and real time RT-PCR detection methods of yellow fever virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Yellow fever virus RT-PCR and real-time fluorescence
RT-PCR detection method
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. SHI Yong Xia, Hong Ye, Ding Guoyun phase Dapeng Sun Wei, Li Xiaobo, Zheng Kui, Huang Ji City, Lu Qin Fortunately, Gobo rotation,
Zhong Yuqing, Li Yan.
Yellow fever virus RT-PCR and real-time fluorescence
RT-PCR detection method
1 Scope
This standard specifies the border crossings to detect yellow fever biosafety requirements, specimen collection, transport and storage, processing specimens, yellow fever
Poison conventional RT-PCR, and real-time RT-PCR detection method.
This standard applies to the border port of entry and exit media personnel mosquitoes carry yellow fever virus detection or molecular biology.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
Human infection of pathogenic microorganisms directory (Ministry of Health)
3 Abbreviations
The following abbreviations apply to this document.
RT-PCR. reverse transcription - polymerase chain reaction
Real-time fluorescent RT-PCR. Real-time fluorescence reverse transcription - polymerase chain reaction
When the number of cycles of each reaction tube fluorescent signal reaches the threshold set by experienced. Ct values
RNA. ribonucleic acid
FAM. FAM fluorescent dye, a fluorescent reporter group
4 Terms and Definitions
The following terms and definitions apply to this document.
4.1
Yellow fever yelowfever
By the Flaviviridae family, genus flavivirus yellow fever virus (yelowfevervirus; YFV) acute infectious disease caused by Aedes aegypti (Aedes
aegypti) it is the major media, is prevalent in Africa and Central and South America, cases March to April more. Clinical features have made
Heat, severe headache, jaundice, bleeding, and proteinuria.
4.2
Yellow fever virus yelowfevervirus
Belongs to the Flaviviridae Flavivirus virus particles are spherical, the diameter of 37nm ~ 50nm, lipoprotein envelope surrounding the outside, coated surface
There are spikes. Viral genome is a single positive-strand RNA, relative molecular mass of about 3.8 × 106, about 11kb.
5 bio-safety requirements
In accordance with GB 19489 and "human infectious pathogens List" request is required for biosafety yellow fever virus related materials as follows.
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