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SN/T 3523-2013 PDF English

Std ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status
SN/T 3523-2013	English	RFQ	ASK	6 days [Need to translate]	Chemicals. Fish assay for oestrogenic and androgenic activity, and aromatase inhibition	Obsolete

Standard similar to SN/T 3523-2013

SN/T 5268 |

Basic data

Standard ID	SN/T 3523-2013 (SN/T3523-2013)
Description (Translated English)	Chemicals. Fish assay for oestrogenic and androgenic activity, and aromatase inhibition
Sector / Industry	Commodity Inspection Standard (Recommended)
Word Count Estimation	40,430
Regulation (derived from)	AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall)
Issuing agency(ies)	General Administration of Customs
Summary	This standard specifies the chemicals on fish estrogen, androgen and methods aromatase inhibitor activity test method described limitations, test principle, test acceptability criteria, methods are described, test design, test procedures, data and reports

SN/T 3523-2013: Chemicals. Fish assay for oestrogenic and androgenic activity, and aromatase inhibition

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Chemicals. Fish assay for oestrogenic and androgenic activity, and aromatase inhibition People's Republic of China Entry-Exit Inspection and Quarantine Standards Chemicals fish estrogen, androgens and aromatase Test method for inhibiting the activity of Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Introduction Ⅲ Introduction Ⅳ 1 Scope 1 2 Terms and definitions 1 Method 3 Limitations Description 1 4 Test Principle 2 5 test acceptability criteria 2 6 Method Description 2 6.1 Instrument 2 6.2 Water 3 6.3 Test Solution 3 6.4 fish feeding 3 6.5 Pre-exposure and selection of fish 3 7 Test Design 4 7.1 General Description 4 7.2 Option 4 concentrations tested 8 Test Procedure 4 8.1 Selection and weighing of test fish 4 8.2 Exposure conditions 4 8.2.1 Time 4 4 8.2.2 Feeding 8.2.3 lighting and temperature 5 8.3 Analysis of frequency detection and measurement of 5 8.4 Observation 5 8.4.1 General Description 5 8.4.2 survival 5 8.4.3 behavior and apparent 5 8.4.4 fish humanely killed 5 8.4.5 Observation of secondary sexual characteristics 6 8.4.6 vitellogenin (VTG) 6 9 data and reports 6 9.1 using analysis of variance (ANOVA) for biomarker response were evaluated 6 9.2 Test results Report 7 10 For the interpretation of the test results and acceptance 8 Recommended sample program in Appendix A (informative) vitellogenin analysis of collected 9 Annex B (informative) detection of specific endocrine active substances for fathead and secondary sexual characteristics of fish medaka assessment 18 Annex C (informative) fish endocrine screening tests experimental conditions 23 Annex D (informative) zebrafish and fathead minnow spawning substrate 25 Annex E (informative) acceptable dilution water some chemical characteristics of 27 Annex F (informative) vitellogenin strengthened between samples and reference standard analytical methods 28 Annex G (informative) Statistical Analysis Process 29 References 30

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard reference Organisation for Economic Co-operation and Development (OrganizationforEconomicCooperationandDevelopment, OECD) TG230. "21 Tian fish test. estrogens, androgens, and aromatase inhibiting activity of short-term screening test" (21-dayFishAssay. A Short-TermScreeningforOestrogenicandAndrogenicActivity, andAromataseInhibition) (in English). This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Chinese Academy of Inspection and Quarantine. The main drafters of this standard. Cheng Yan, Cui Yuan, Falls Apart, Xie Wenping, Ma Ping.

Introduction

Ambient levels of chemical interaction with the endocrine system, crimes against humanity and wildlife, so the need to develop and validate detection within fish Secretion interference techniques and methods of active substances. In 1998, OECD began the process of revising the existing test guidelines and the development of new testing guidelines, To screen and detect potential endocrine disruptors. One of the important research is the development of test methods in fish endocrine disruptors guide. 21 days in fish endocrine disruptor screening test (TG230) after extensive validation tests, including for the selected chemicals, the three fish Germline (fathead minnow, Japanese medaka and zebrafish) on the biological model, about estrogen and aromatase across multiple laboratories inhibiting substance ([1] to [5]) method to test the relevance and reliability of analytical testing; Androgen tests can be spent on fish and medaka model in black, but can not Performed on zebrafish model. Test Guidelines (TG230) does not apply to testing antiandrogen. Test method validation guidelines set by the NCBs (theNationalCoordinatorsoftheTestGuidelineProgramme) specified by the Group of Experts to complete [6]. This test does not identify the specific mechanisms of hormonal disturbance, because the animals tested has a complete hypothalamic - pituitary - gonadal (HPG) axis, will shadow Substance ring HPG axis have different degrees of reaction. Fish short-term reproductive toxicity test (TG229) including fertility and fathead minnow (in Where appropriate) gonadal tissue was observed, and in this test (TG230) included in all test endpoints. TG229 provides through Through different mechanisms (including endocrine features) method for screening chemicals affect reproductive capacity. This is in choosing the most appropriate testing guidelines When priority. This test method describes a method for in vivo screening test, sexually mature males and females in the spawning period together, in their lives Period of exposure to chemicals for a limited time (21 days). When the 21-day exposure period ends, according to the male and female fish species used, On for one or two biomarkers endpoints tested as estrogen test chemicals, aromatase inhibiting or androgen activity refers to Mark. These tests include the end of vitellogenin and secondary sexual characteristics. For fathead minnow, Japanese medaka and zebrafish can be vitellogenin Test, and secondary sexual characteristics can only stay on the Japanese medaka fish and test blackheads. This biological test as a specific pattern of endocrine disruptors in vivo Screening test, its application can be seen in "OECD endocrine-disrupting chemicals testing and evaluation framework" on. Chemicals fish estrogen, androgens and aromatase Test method for inhibiting the activity of

1 Scope

This standard specifies the chemicals on fish estrogen, androgen and methods aromatase inhibiting activity test the limitations of the method described, the original test Management, test acceptability criteria, methods are described, test design, test procedures, data and reports for the interpretation of the test results and acceptance. This standard applies to chemicals in fish estrogens, androgens, and aromatase inhibiting activity tests.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Load factor loadingrate Unit volume of water the fish wet weight. 2.2 Carrier density stockingdensity The number of fish per volume of water. 2.3 Vitellogenin vitelogenin, VTG Vitellogenin is a phospholipid glycoprotein precursor for forming a yolk protein, generally produced in all oviparous animals sexually mature female body. 2.4 The maximum tolerated concentration maximumtoleratedconcentrationMTC The maximum tolerated concentration, represents about 10% of the LC50. 2.5 HPG axis hypothalamic-pituitary-gonadalaxis Hypothalamic - pituitary - gonadal axis. Method 3 Limitations Description 3.1 vitellogenin (Vitelogenin, VTG) normally circulating endogenous estrogen hormone stimulation that, by the female egg-laying vertebrates liver produce. Yolk protein vitellogenin is a prerequisite, once produced in the liver by blood flow into the ovaries and developing female gamete suck Income and change. Vitellogenin in immature female and male fish was barely detectable in plasma, because they lack sufficient circulating estrogen Hormones, but the stimulation by exogenous estrogen, the liver can synthesize and secrete VTG. 3.2 VTG measurements can detect different modes of action of estrogen chemicals detected estrogenic chemicals, it is possible through Measurement of vitellogenin in male fish produce, and there have been numerous reports [7] in the scientific literature counterparts. Also there is evidence showing vitellogenin Produce can be caused by exposure to the aroma of androgens [8] - [9]. Lowering the female body in circulating levels of estrogen, e.g., by inhibiting Conversion of endogenous androgens to estrogens 17β- estradiol natural aromatase enzyme, causing reduced levels of vitellogenin, which is used to check Having aromatase inhibiting properties measured chemicals [10] to [11]. It has been established in response to vitellogenin estrogen/aromatase inhibition Biological relevance, and it has been widely reported in the literature. However, in the female body VTG generated by the system it may also have a non-toxic and within Secretion interference influence toxicity models, such as liver toxicity.