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SN/T 3506-2013 PDF English

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US$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 3506-2013: Procedure of fluorescence polarisation assay for brucellosis
Status: Obsolete

Std ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status
SN/T 3506-2013	English	239	Add to Cart	3 days [Need to translate]	Procedure of fluorescence polarisation assay for brucellosis	Obsolete

Standard similar to SN/T 3506-2013

SN/T 5268 |

Basic data

Standard ID	SN/T 3506-2013 (SN/T3506-2013)
Description (Translated English)	Procedure of fluorescence polarisation assay for brucellosis
Sector / Industry	Commodity Inspection Standard (Recommended)
Word Count Estimation	6,631
Quoted Standard	GB/T 6682; GB 19489
Regulation (derived from)	?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165)
Issuing agency(ies)	General Administration of Customs
Summary	This standard specifies the brucellosis fluorescence polarization test technical requirements. This standard applies to brucellosis quarantine, screening and monitoring.

SN/T 3506-2013: Procedure of fluorescence polarisation assay for brucellosis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Procedure of fluorescence polarisation assay for brucellosis People's Republic of China Entry-Exit Inspection and Quarantine Standards Brucellosis Fluorescence Polarization test procedures Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Wang Yuling, Shun Shen Luan, Chai Chun-ming. Brucellosis Fluorescence Polarization test procedures

1 Scope

This standard specifies the fluorescence polarization brucellosis test technical requirements. This standard applies to brucellosis quarantine, screening and monitoring.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety

3 Biosecurity

In order to protect the safety of laboratory personnel, the staff should be trained through biosafety detect brucellosis should be taken Aerosol infection prevention safety measures. All waste should be disposed of in accordance with the relevant provisions of GB 19489.

4 Test principle

Molecules in solution in random rotation. Size of the molecule is the main factor affecting the rate of rotation, and its inverse relationship. Thus the small hours Sub larger molecules to rotate faster. If the marker on a fluorescent dye, by the time the rotation angle of 68.5 ° can be measured by the level and Vertical polarized light intensity is determined. Thus, small molecule rotating faster than the molecules emitted more polarized. When the diagnosis of brucellosis, Brucella smooth type LPS small molecular weight fragments OPS (average 22kD), marked with fluorescence isothiocyanate Su (FITC), used as an antigen. Then added to the serum to be tested, and if it will reduce its rotational speed antibody and labeled antigen binding with fluorescent Light polarizing instrument measured polarization values determined overcast samples by positive polarization values.

5 Reagents and materials

5.1 Positive control. Fluorescence polarization brucellosis Commercially available reagents. 5.2 negative control; brucellosis fluorescence polarization commercialized reagents. 5.3 25 × concentrated reaction buffer. Brucellosis fluorescence polarization commercialized reagents. 5.4 conjugate. labeled with fluorescein isothiocyanate O-polysaccharide (OPS) antigen, fluorescence polarization brucellosis Commercially available reagents. 5.5 96 well black flat bottom microplates. 5.6 Unless otherwise specified, this method should be used three water test water line with GB/T 6682 regulations.

6 instruments and equipment

6.1 fluorescence polarization instrument.