Path:
Home >
SN/T >
Page61 > SN/T 3496-2013
|| Home >
Standard_List >
SN/T >
Page61 > SN/T 3496-2013
Price & Delivery
US$319.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 3496-2013: Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
Status: Valid
| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 3496-2013 | English | 319 |
Add to Cart
|
3 days [Need to translate]
|
Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
| Valid |
Standard similar to SN/T 3496-2013
HG/T 2935 | SN/T 1353 | HG 2934 | SN/T 5657 | SN/T 5116 | SN/T 3497 | Basic data
| Standard ID | SN/T 3496-2013 (SN/T3496-2013) |
| Description (Translated English) | Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 12,192 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the method for real-time PCR test GMO animal feed. This standard applies to pigs, cattle, sheep genetically modified animal feed real-time PCR test. |
SN/T 3496-2013: Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the real-time PCR for detecting genetically modified component in animal-originated-feedstuffs
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Genetically modified animal feed real-time fluorescence
PCR detection method
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Anhui Exit Inspection and Quarantine, the People's Republic of China
And country SZCIQ, China Agricultural University, Beijing Animal Husbandry and Veterinary.
The main drafters of this standard. Liu, Linxiang Mei, ZHU Zhen camp, Zhang Yongning, Jianwu, Wu Shaojiang, Zhenghai Song Ling apricot, Xue Zhenhua, Zhang Min,
Yu Ning, Caixia, Li Zhihui, Li Jinghong.
Genetically modified animal feed real-time fluorescence
PCR detection method
1 Scope
This standard specifies the method for real-time PCR testing of genetically modified animal feed.
This standard applies to pigs, cattle, sheep, animal feed GMO real time PCR test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
β2m. β2-microglobulin (β2-microglobulin)
bp. base pairs (basepair)
CMV. CMV (cytomegalovirus)
Ct. each reaction cycle number (cyclethreshold) fluorescence signal within the tube reaches the set threshold experienced
dATP. triphosphate (deoxyadenosinetriphosphate)
dCTP. deoxycytidine triphosphate (deoxycyticinetriphosphate)
dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate)
dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate)
dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate)
DNA. deoxyribonucleic acid (deoxyribonucleicacid).
GFP. green fluorescent protein (greenfluorescentprotein)
NPTⅡ. neomycin 3'-phosphotransferase gene (neomycin-3'-phosphotransferasegene)
PCR. polymerase chain reaction (polymerasechainreaction)
SV40. simian virus 40 (SimianVacuolatingvirus40)
Taq DNA. DNA polymerase.
TE. Tris-HCl, EDTA buffer
Tris. Tris (hydroxymethyl) aminomethane (trishydroxymethylaminomethane)
4 Experimental Method
4.1 The main instruments
4.1.1 Real-Time PCR instrument.
4.1.2 clean bench.
4.1.3 Sterilization pot.
4.1.4 Vortex.
Tips & Frequently Asked Questions:
Question 1: How long will the true-PDF of SN/T 3496-2013_English be delivered?
Answer: Upon your order, we will start to translate SN/T 3496-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.
Question 2: Can I share the purchased PDF of SN/T 3496-2013_English with my colleagues?
Answer: Yes. The purchased PDF of SN/T 3496-2013_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.
Question 3: Does the price include tax/VAT?
Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs):
List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?
Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.