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SN/T 3433-2012: Detection and identification of Rigidoporus lignosus (Klotzsch) Imaz.
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Detection and identification of Rigidoporus lignosus (Klotzsch) Imaz.
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Standard similar to SN/T 3433-2012 SN/T 5268 | Basic data | Standard ID | SN/T 3433-2012 (SN/T3433-2012) | | Description (Translated English) | Detection and identification of Rigidoporus lignosus (Klotzsch) Imaz. | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 15,170 | | Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the rubber immigration phytosanitary Shirane germs Rigidoporus lignosus (Klotzsch) Imaz. Quarantine identification. This standard applies to the case of Hevea rubber plants such as seedlings and other propagating material rubber Sh | SN/T 3433-2012: Detection and identification of Rigidoporus lignosus (Klotzsch) Imaz.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Rigidoporus lignosus (Klotzsch) Imaz.
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Rubber Shirane quarantine and identification of bacteria
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard is drafted by. People's Republic of China Hainan Entry-Exit Inspection and Quarantine Bureau.
Participated in the drafting of this standard. People's Republic of China, Ningbo Entry-People's Republic of China Guangdong Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Han Yuchun, Liu Fuxiu, Li Weidong, Xu Wei, Lin Mingguang, Zhao Lirong, Wen Weigang.
Rubber Shirane quarantine and identification of bacteria
1 Scope
This standard specifies the immigration phytosanitary rubber white roots germs Rigidoporuslignosus (Klotzsch) Imaz. Identification of quarantine
method.
This standard applies to the case of quarantine and identification of the Hevea rubber plants such as seedlings or other propagating materials rubber Shirane bacteria.
Principle 2
Scientific name. Rigidoporuslignosus (Klotzsch) Imaz.
Synonyms. Fomeslignosus (Klotzsch) Bres.
Rigidoporusmicroporus (Fr.) Overeem
PolyporuslignosusKlotzsch
Rubber white roots germs Rigidoporuslignosus (Klotzsch) Imaz, belonging to Fungi Fungi, Basidiomycota Basidiomycota,
Basidiomycetes Basidiomycetes, no separated Basidiomycetes subclass Holobasidiomycetidae, non-pleated mushroom head Aphylophorales, thin volvatus
Section Meripilaceae, rigidoporus Rigidoporus fungi.
Rubber white root root bacteria genus obligate dwelling bacteria, mainly rubber genus parasitic plant roots, leaving the host organization can not survive in the soil.
The bacteria in infected plant process can produce varying thickness rhizomorph, fruiting root lesion wet weather. The bacteria
Mainly through root contact by rhizomorph spread spread it can also be produced by the fruiting bodies through airborne spores settled to the stumps or root section
Department of the wound to form a new source of infection, through root contact. Damage symptoms of the pathogen, morphological, cultural traits, and Pathogenicity Test
Identification of molecular biology information is based on white rubber root bacteria. For details refer to Appendix A and Appendix B.
3 Reagents and culture medium
3.1 Reagents
Oatmeal agar, sucrose.
Trihydrocarbyl aminomethane hydrochloride, cetyl trimethyl ammonium bromide, polyvinylpyrrolidone, EDTA, proteinase K,
Glacial acetic acid, bromophenol blue, sodium chloride, chloroform, isoamyl alcohol, isopropyl alcohol, DNA molecular weight Marker, ethidium bromide, sodium hypochlorite.
10 × PCR buffer, magnesium chloride, dNTP, TaqDNA polymerase, the PCR primers, agarose.
3.2 Medium
Bacteria isolated and purified using oatmeal agar medium (see Appendix C).
Oatmeal agar plates. the sterile oatmeal agar medium was poured into sterile petri dishes melted to form 2mm ~ 3mm thick flat plates.
Oatmeal agar slant. A 2mL ~ 4mL oatmeal agar medium in a test tube, and after sterilization, the tubes were immediately placed in a certain slope
On the slope, after solidification beveled media.
4 apparatus and appliances
4.1 Instrument
PCR instrument, clean benches, autoclaves, ice maker, nucleic acid protein analyzer, high-speed refrigerated centrifuge, small desktop centrifuge, ultra
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