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SN/T 3402-2012: Identification genuineness and purity of two-line rice varieties using DNA analysis method
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Identification genuineness and purity of two-line rice varieties using DNA analysis method
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Standard similar to SN/T 3402-2012 SN/T 5268 | Basic data | Standard ID | SN/T 3402-2012 (SN/T3402-2012) | | Description (Translated English) | Identification genuineness and purity of two-line rice varieties using DNA analysis method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 17,147 | | Quoted Standard | GB/T 3543.2 | | Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the rice light, temperature sensitive genic male sterile line and two-line hybrid rice varieties authenticity and purity analysis of SSR markers. This standard applies to rice light, temperature sensitive genic male sterile line an | SN/T 3402-2012: Identification genuineness and purity of two-line rice varieties using DNA analysis method
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Identification genuineness and purity of two-line rice varieties using DNA analysis method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Authenticity and Purity Testing of Two - line Rice Varieties
DNA analysis
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Anhui Entry-Rice Research Institute, Anhui Academy of Agricultural Sciences, the Chinese people
Hunan People's Republic of Exit Inspection and Quarantine.
The main drafters of this standard. Yang Jianbo, Yao Jian, Lu Xu Zhong, Ni Jinlong, Chen Xuejiao, Zhenghai Song, Li Yunfei, Zhang Ping, Zhang Jian, the Xi Han,
Liuhai Zhen, Zhang Xiaojuan, Ma Lin, Zhu Jinguo.
Authenticity and Purity Testing of Two - line Rice Varieties
DNA analysis
1 Scope
This standard specifies the method of rice SSR markers light PTGMS and two-line hybrid rice varieties authenticity and purity.
This standard applies to rice light, PTGMS identification and authenticity and purity of hybrid rice varieties.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 3543.2 agricultural seed testing protocols for sampling
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Rice light, PTGMS ricephotoperiod/thermo-sensitivegenicmalesterileline
Also known as the two CMS lines, the use of rice light, temperature sensitive genic male sterile rice male sterile line breeding characteristics, its tables under short-day conditions of low temperature
Now male fertility, can be propagated seed; long day at high temperature performance of male sterility, can be used to seed.
3.2
Two-line hybrid rice two-linehybridrice
Rice Using light, PTGMS and restored hybrids produce F1 generation.
3.3
Two - line Rice two-line-rice
This standard will be two-line hybrid rice sterile line and two line system called two rice.
3.4
Microsatellite markers simplesequencerepeat (SSR) marker
By a few nucleotides (generally 2 to 6) as a tandem repeat unit of tens to hundreds of times as many as the repeat; the basic unit of repetitions
Different numbers, thus the formation of polymorphic SSR loci; a pair of specific primers based on SSR on both sides of the seat single-copy conserved sequence spread
By SSR sequence, you can reveal the polymorphism.
3.5
Standard sample standardsample
The sample was representative of the certification authority finds that the known characteristics of the variety of species, sexual reproduction, usually seeds.
Principle 4
China's production line on two sterile transgenic rice mainly PSGMS gene pms1, pms3 and temperature sensitive genic male sterile gene tms5,
The corresponding molecular markers characteristic of pms1-S1, pms3-C1, tms5-S2, based on these markers characteristic can be determined whether the two water lines
Rice; while taking advantage of the rice genome, there are a lot of differences in the number of repeat units SSR among different species, divided by SSR Polymorphism
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