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SN/T 3399-2012: Detection and identification of Diaporthe phaseolorum var.caulivora and Diaporthe phaseolorum var.meridionalis. Real-time fluorescent PCR with Taq Man MGB probe
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| SN/T 3399-2012 | English | 159 |
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Detection and identification of Diaporthe phaseolorum var.caulivora and Diaporthe phaseolorum var.meridionalis. Real-time fluorescent PCR with Taq Man MGB probe
| Valid |
Standard similar to SN/T 3399-2012 SN/T 5268 | Basic data | Standard ID | SN/T 3399-2012 (SN/T3399-2012) | | Description (Translated English) | Detection and identification of Diaporthe phaseolorum var.caulivora and Diaporthe phaseolorum var.meridionalis. Real-time fluorescent PCR with Taq Man MGB probe | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 6,619 | | Quoted Standard | SN/T 1899 | | Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the soybean stem canker include soy northern stem canker [Diaporthe phaseolorum (Cooke et Ell.) Sacc. Var. Caulivora Athow et Caldwell] Southern stem canker and soybean [Diaporthe phaseolorum (Cooke et Ell.) Sacc. Var. meridionalis | SN/T 3399-2012: Detection and identification of Diaporthe phaseolorum var.caulivora and Diaporthe phaseolorum var.meridionalis. Real-time fluorescent PCR with Taq Man MGB probe
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Detection and identification of Diaporthe phaseolorum var.caulivora and Diaporthe phaseolorum var.meridionalis.Real-time fluorescent PCR with Taq Man MGB probe
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Soybean stem canker quarantine and identification of TaqMan
MGB probe real-time PCR detection method
phaseolorumvar.meridionalis-Real-timefluorescentPCRwithTaqManMGB probe
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute.
The main drafters of this standard. Chen Chi-nan, Wang Ying, Cheng Yinghui Hu Xian Xing, Zhang Guiming.
Soybean stem canker quarantine and identification of TaqMan
MGB probe real-time PCR detection method
1 Scope
This standard specifies the soybean stem canker, including soybeans northern stem canker [Diaporthephaseolorum (CookeetEl.) Sacc.
var.caulivoraAthowetCaldwel] Southern stem canker and soybean [Diaporthephaseolorum (CookeetEl.)
Sacc.var.meridionalisF.A.Fernandez] TaqManMGB time PCR probe quarantine and identification methods.
This standard applies to soybean seeds, plant debris soybean stem canker quarantine and identification of real-time PCR.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1899 Phytosanitary soybean stem canker quarantine and identification methods
Principle 3
3.1 Basic Information
scientific name.
Soybean stem canker North. Diaporthephaseolorum (CookeetEl.) Sacc.var.caulivoraAthowetCald-
wel
Southern soybean stem canker. Diaporthephaseolorum (CookeetEl.) Sacc.var.meridionalisF.A.Fer-
nandez
English common name. SoybeanStemCanker
Taxonomic position. Soybean stem canker fungi industry Fungi, Ascomycota Ascomycota, Ascomycetes steel Ascomycetes, Zama shell
Head Diaporthales, black shell Yuba Branch Valsaceae, Zama shell genus DiaportheNits.
Approximate species. Soybean stem blight pod rot pathogen Diaporthephaseolorumvar.sojae and soybeans Phomopsis species Phomopsis rot pathogen
longicolla.
3.2 Identification of principle
According to molecular biological characteristics of soybean northern and southern stem canker and real-time PCR principles, including real-time application related instruments fluorescence
Light PCR instrument, such as soybean northern and southern stem canker were identified.
4 appliances, instruments and reagents
4.1 Instrument appliances
Ovens, autoclaves, microscopes, dissecting microscope, the balance (the amount of sense 1/100; 1/10000), mortar, shaker, water bath, ice machine, water
Instrument, Vortex, desktop centrifuges, nucleic acid protein analyzer, high-speed refrigerated centrifuge, vacuum drained instrument, refrigerator, clean benches, real-time fluorescence
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