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SN/T 3027-2011: Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay
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| SN/T 3027-2011 | English | 389 |
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Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay
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SN/T 3027-2011
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Basic data | Standard ID | SN/T 3027-2011 (SN/T3027-2011) | | Description (Translated English) | Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B47 | | Classification of International Standard | 65.140 | | Word Count Estimation | 15,171 | | Date of Issue | 2011-09-09 | | Date of Implementation | 2012-04-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 492 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of enzyme-linked immunoassay royal jelly Buddha quinolone residues. This standard applies to royal jelly nadifloxacin, rufloxacin, Danofloxacin, enrofloxacin, ciprofloxacin, levofloxacin, ofloxacin, Pefloxacin, norfloxacin, two Sarafloxacin determination and total residual enoxacin, difloxacin. | SN/T 3027-2011: Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay
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Determination of fluoroquinolones residues in royal jelly for export.Enzyme-linked immunosorbent assay
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of fluoroquinolone residues in royal jelly export
ELISA
Issued on. 2011-09-09
2012-04-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
In accordance with this standard GB/T 1.1-2009 rules drafted.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters. Zhang Xiaofeng, Zhu Zhenjiang, Wu Shan, Shuai Jiang Bing, Chen Xiaomei.
Determination of fluoroquinolone residues in royal jelly export
ELISA
1 Scope
This standard specifies the method of enzyme-linked immunosorbent assay Fluoroquinolones Residues in Royal Jelly.
This standard applies to royal jelly nadifloxacin, rufloxacin, danofloxacin, enrofloxacin, ciprofloxacin, levofloxacin, oxyfluoride
Gatifloxacin, measured pefloxacin, norfloxacin, difloxacin, enoxacin sarafloxacin and residual total.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Perchloric acid to precipitate proteins of royal jelly and royal jelly extract residues fluoroquinolones. Microplate is coated with a fluorine Connaught sand
Star antibody binding horseradish peroxidase was added norfloxacin (norfloxacin enzyme-labeled), norfloxacin after standard or sample extract, standard
Norfloxacin solution or sample and enzyme-labeled fluoroquinolones norfloxacin norfloxacin competitively bind to the antibody. By washing
Remove unbound reagent, then adding chromogenic substrate, absorbance was measured using a microplate reader, the absorbance of the sample is worth fluoroquinolones
content.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
Fluoroquinolone 4.1 Randox test kits 1).
1) This information is given for the convenience of users of this standard does not imply endorsement of a particular product. If other product has the same effect, subject to real
Using these products is equivalent to a posteriori assessment.
4.2 norfloxacin enzyme conjugate solution. according to kit instructions fluoroquinolones, dilution with enzyme labels (see Appendix A) the fluorine Connaught
Shaxing enzyme conjugate concentrate (see Appendix A) was dissolved, mix and reserve.
Dilution 4.3 wash buffer. fluoroquinolones kit according to instructions, the diluted wash buffer concentrate (see Appendix A)
Add 970mL diluted with water, after mixing spare.
4.4 phosphoric acid. AR.
4.5 NaCl. AR.
4.6 methanol. AR.
4.7 30% sodium hydroxide. 30gNaOH dissolved in water, water volume to 100mL.
4.8 6% perchloric acid. Take 6mL perchlorate volume with water to 100mL.
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