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SN/T 2973-2011: Quarantine protocol for xenohaliotis californiensis
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Quarantine protocol for xenohaliotis californiensis
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Standard similar to SN/T 2973-2011 GB 5009.261 | SC/T 4030 | Basic data | Standard ID | SN/T 2973-2011 (SN/T2973-2011) | | Description (Translated English) | Quarantine protocol for xenohaliotis californiensis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B57 | | Classification of International Standard | 65.020 | | Word Count Estimation | 15,149 | | Date of Issue | 2011-09-30 | | Date of Implementation | 2012-04-01 | | Quoted Standard | GB/T 18088 | | Adopted Standard | OIE Aquatic Animal Disease Diagnostic Manual -2009, NEQ | | Regulation (derived from) | National Quality Inspection (2011) 492; industry standard filing Notice 2012 No. 4 (No. 148 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the abalone rickettsial disease histopathology, PCR and in situ hybridization method quarantine technical specifications. This standard applies to abalone rickettsial disease, routine testing for the disease epidemiology, diagnosis, quarantine and monitoring. | SN/T 2973-2011: Quarantine protocol for xenohaliotis californiensis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for xenohaliotis californiensis
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Abalone rickettsial disease quarantine Technical Specifications
Issued on. 2011-09-09
2012-04-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard and the International Organization for Animal Health (OIE) "aquatic animal disease diagnostic manual" (2009 edition) in chapter 2.4.7 "Infection
withXenohaliotisCaliforniensis "non-equivalent.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine of Liaoning People's Republic of China
Bureau, the Shenzhen People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Yue Zhiqin, Zhao Ran, Wu Bin, Liu Orientin, Li Ye, Xinxue Qian, Shixiu Jie, Su Yongsheng, Liang beads.
Abalone rickettsial disease quarantine Technical Specifications
1 Scope
This standard specifies the histopathological observation abalone rickettsial disease, PCR and in situ hybridization quarantine technical specifications.
This standard applies to routine testing abalone rickettsial disease, epidemiological investigation applicable to this disease, diagnosis, quarantine and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 equipment and reagents
3.1 device
3.1.1 ordinary optical microscope.
3.1.2 -80 ℃ cryogenic refrigerator.
3.1.3 tissue grinder.
3.1.4 slicer.
3.1.5 PCR instrument.
3.1.6 electrophoresis.
3.1.7 gel imager or ultraviolet light observation.
3.2 Reagents
3.2.1 stain. Conventional smears and histological stain (about dye Appendix A).
3.2.2 proteinase K (see Appendix A).
3.2.3 paraffin.
3.2.4 xylene.
3.2.5 ethanol.
3.2.6 75% ethanol.
3.2.7 magnesium sulfate (MgSO4).
3.2.8 dNTP.
3.2.9 TaqDNA polymerase and PCR buffer (Appendix A).
3.2.10 DNA2000Marker.
3.2.11 nucleic acid dye (EB).
3.2.12 agarose.
3.2.13 Davidson's fixative (Appendix A).
3.2.14 PCR product purification kit.
3.2.15 digoxin labeling kit.
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