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SN/T 2867-2011: Qualitative identification of fish derived material in feedstuff. PCR method
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| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 2867-2011 | English | 209 |
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Qualitative identification of fish derived material in feedstuff. PCR method
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Standard similar to SN/T 2867-2011
HG/T 2935 | SN/T 1353 | HG 2934 | SN/T 5657 | SN/T 5123 | SN/T 5116 | Basic data
| Standard ID | SN/T 2867-2011 (SN/T2867-2011) |
| Description (Translated English) | Qualitative identification of fish derived material in feedstuff. PCR method |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Word Count Estimation | 8,810 |
| Date of Issue | 2011-05-31 |
| Date of Implementation | 2011-12-01 |
| Quoted Standard | GB/T 6682; GB/T 14699.1; SN/T 1193 |
| Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 291 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the method of PCR-derived ingredients of fish feed detection. The detection limit of the assay was 0. 1%. This standard applies to the qualitative detection of fish feeds derived ingredients. |
SN/T 2867-2011: Qualitative identification of fish derived material in feedstuff. PCR method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Qualitative identification of fish derived material in feedstuff.PCR method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Fish derived materials in feed detection method
PCR methods
PCRmethod
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau.
The main drafters. Zhang Shuya, bear Wei, Jiang Jing, Pan Liangwen.
Fish derived materials in feed detection method
PCR methods
1 Scope
This standard specifies the method of PCR-derived ingredients fish feed detection, detection limit of the assay was 0.1%.
This standard applies to qualitative detection of fish feed-derived ingredients.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699.1 feed samples
SN/T 1193 genetic testing laboratory technical requirements
3 Abbreviations
PCR (polymerasechainreaction). Polymerase chain reaction
DNA (deoxyribonudeicacid). DNA
Principle 4
Use of cell lysate broken, chloroform extraction of proteins, ethanol precipitation to give DNA; with the extracted DNA as a template
PCR amplification and agarose gel electrophoresis to detect PCR amplification products; positive PCR product was sequenced application for confirmation.
5 Reagents and materials
Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Test water line with GB/T 6682 requirements. All reagents were
Pollution-free DNA enzyme dispensing container.
5.1 detection primer comprising.
a) Forward primer. 5'-TAAgAgggCCggTAAAACTC-3 '
b) reverse primer. 5'-gTggggTATCTAATCCCAg-3 '
5.2 cetyl trimethyl ammonium bromide (CTAB).
5.3 Tris (Tris).
5.4 ethylenediaminetetraacetic acid (EDTA).
5.5 Sodium chloride (NaCl).
5.6 chloroform (chloroform).
5.7 isopropanol.
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