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SN/T 2850-2011: Quarantine protocol for viral haemorrhagic septicaemia (VHS)
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| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 2850-2011 | English | 339 |
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Quarantine protocol for viral haemorrhagic septicaemia (VHS)
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Basic data
| Standard ID | SN/T 2850-2011 (SN/T2850-2011) |
| Description (Translated English) | Quarantine protocol for viral haemorrhagic septicaemia (VHS) |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 13,111 |
| Date of Issue | 2011-02-25 |
| Date of Implementation | 2011-07-01 |
| Quoted Standard | GB/T 6682; GB/T 18088 |
| Regulation (derived from) | ?AQSIQ-Inspection [2011] 90 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the viral hemorrhagic septicemia virus (viral haemorrhagic septicaemia virus, VHSV) detected by cells isolated virus by indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), reverse polymerization country enzyme chain reflects lower than (PT-PCR) and sequencing identified quarantine norms. This standard applies to diagnostic and epidemiological investigation of fish quarantine viral hemorrhagic septicemia. |
SN/T 2850-2011: Quarantine protocol for viral haemorrhagic septicaemia (VHS)
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for viral haemorrhagic septicaemia (VHS)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Viral hemorrhagic septicemia quarantine Technical Specifications
Issued on. 2011-02-25
2011-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard reference to the OIE (OIE) "aquatic animal disease diagnostic manual 2009 Edition" 2.3.9 Chapter viral haemorrhagic
Sepsis method.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China SZCIQ, People's Republic of China Beijing Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. High-Long Ying, Zhang Lifeng, Duan An, Shixiu Jie, Xu Ye, Jiang Yu-lin.
Viral hemorrhagic septicemia quarantine Technical Specifications
1 Scope
This standard specifies the viral hemorrhagic septicemia virus (viralhaemorrhagicsepticaemiavirus, VHSV) detected by the
Cells isolated from the virus, the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), reverse transcriptase polymerase chain reaction
(RT-PCR) and sequencing identified quarantine norms.
This standard applies to fish quarantine diagnosis and epidemiological investigation of viral hemorrhagic septicemia.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Abbreviations
The following abbreviations apply to this document.
BF-2. bluegill cell lines
CPE (cytopathiceffect). cytopathic effect
DEPC. diethyl pyrocarbonate
dNTP (deoxyribonucleosidetriphosphate). deoxyribonucleoside triphosphate
EDTA (ethylenediaminetetraaceticacid). ethylenediaminetetraacetic acid
EPC. Carp epithelial cell line
FCS (fetalcalfserum). FBS
HEPES. N-2- hydroxyethyl piperazine-ethanesulfonic acid -N'-2-
RTG-2. rainbow trout gonadal cells
RT-PCR (reversetranscription-polymerasechainreaction). reverse transcriptase polymerase chain reaction
Taq enzyme (TaqDNApolymerase). TaqDNA polymerase
Tris [tris (hydroxymethyl) aminomethane]. tris (hydroxymethyl) aminomethane
VHSV (ViralHaemorrhagicSepticaemiavirus). viral hemorrhagic septicemia virus
4 instruments and equipment
4.1 Clean Benches.
4.2 incubator.
4.3 inverted fluorescence microscope.
4.4 refrigerated centrifuge.
4.5 -80 ℃ ultra-low temperature freezer.
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