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SN/T 2727-2010: Determination of poultry derived materials feedstuff. Real-time fluorescent PCR method
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| SN/T 2727-2010 | English | 259 |
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Determination of poultry derived materials feedstuff. Real-time fluorescent PCR method
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SN/T 2727-2010
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Basic data | Standard ID | SN/T 2727-2010 (SN/T2727-2010) | | Description (Translated English) | Determination of poultry derived materials feedstuff. Real-time fluorescent PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B46 | | Classification of International Standard | 65.120 | | Word Count Estimation | 10,190 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682; GB/T 14699; GB/T 27025; SN/T 0800.1 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the feed poultry-derived ingredients (including chickens, ducks, geese, quail), real-time PCR detection method. This standard applies to poultry feed derived ingredients (including chickens, ducks, geese, quail) the qualitative detection. | SN/T 2727-2010: Determination of poultry derived materials feedstuff. Real-time fluorescent PCR method
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Determination of poultry derived materials feedstuff.Real-time fluorescent PCR method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Methods for detection of avian-derived feed
Real-time PCR method
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Yufeng Qin, Jin-Ling Wang, Jiang Li, Wang Fang, Bi Yingjie, Cheng Chiu, Zhang Ying, Mengxiang Yong, PANG Jie.
Methods for detection of avian-derived feed
Real-time PCR method
1 Scope
This standard specifies the poultry feed derived ingredients (including chickens, ducks, geese, quail) of real-time PCR detection method.
This standard applies to poultry feed derived ingredients (including chickens, ducks, geese, quail) qualitative detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 14699 feed sampling method
GB/T 27025 testing and calibration laboratories General requirements
SN/T 0800.1 and export grain, feed inspection sampling and sample preparation methods
3 Terms, definitions and abbreviations
3.1 Definitions and Terminology
The following terms and definitions apply to this document.
3.1.1
Real-time fluorescence PCR real-timefluorescentPCR
Real time polymerase chain reaction.
3.1.2
Ct values cyclethresholdCt
The number of cycles when fluorescence signal of each reaction tube reaches a set threshold experienced.
3.2 Acronyms
The following abbreviations apply to this document.
FAM (6-carboxyfluorescein). 6- carboxyfluorescein
TAMRA (6-carboxytetramethylrhodamine). 6- carboxy-tetramethylrhodamine
4 Sampling and sample preparation
Feed samples collected with reference to GB/T 14699, SN/T 0800.1 performed. 50g solid sample was crushed by a 120 mesh sieve,
Maintaining 0.125mm or less in particle size.
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