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SN/T 2717-2010: Quarantine protocol for equine infectious anemia
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| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 2717-2010 | English | 259 |
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Quarantine protocol for equine infectious anemia
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Basic data
| Standard ID | SN/T 2717-2010 (SN/T2717-2010) |
| Description (Translated English) | Quarantine protocol for equine infectious anemia |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 10,129 |
| Date of Issue | 2010-11-01 |
| Date of Implementation | 2011-05-01 |
| Quoted Standard | GB/T 6682; GB/T 17494 |
| Regulation (derived from) | National Quality Inspection (2010) 582 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the clinical diagnosis of equine infectious anemia virus isolation and identification, enzyme-linked immunosorbent assay, agar gel immunodiffusion test and other quarantine. This standard applies to equine infectious anemia quarantine and diagnostics. |
SN/T 2717-2010: Quarantine protocol for equine infectious anemia
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for equine infectious anemia
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Equine infectious anemia and Quarantine Technical Specifications
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong CIQ.
The main drafters of this standard. China Zhu Liang beads, Zheng Xiaolong, Deng Mingjun, Xiao Xi Zhi, Yue Zhiqin, Xinxue Qian, Sun Tao, Wang Qun.
Equine infectious anemia and Quarantine Technical Specifications
1 Scope
This standard specifies the clinical diagnosis of equine infectious anemia virus isolation and identification, enzyme-linked immunosorbent assay, agar gel immunodiffusion test
Quarantine inspection and other technologies.
This standard applies to equine infectious anemia quarantine and diagnosis.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 17494 indirect ELISA Equine Infectious Anemia Disease Diagnosis
3 clinical diagnosis
3.1 Clinical features
Equine infectious anemia (EIA) characteristic symptoms and pathological changes missed high fever (39 ℃ above), or intermittent fever (high fever 3d ~
5d, by 3d ~ 15d room temperature intermittent relapse), or the difference in temperature inversion phenomenon occurs; the base of the tongue, mouth, nose, vaginal mucosa and conjunctival
At common bright red to dark red bleeding points (spots) and the like; symptoms and pathological changes characteristic of anemia, jaundice, heart weakness, edema and emaciation.
3.2 determination
According to the characteristic symptoms and pathological changes (see 3.1) can make a preliminary diagnosis. Further confirmed disease should be collected serum samples or solid material
Laboratory examination.
4 laboratory tests
4.1 Isolation and identification of viruses
4.1.1 Test material
Cell culture flasks (80mL), Saybolt fluid, cell growth medium, Apgar liquid, donkey WBC preparation see Appendix A. Laboratory water Press
According to GB/T 6682 regulations.
4.1.2 Disease material acquisition and processing
Sterile collection was seized equine anticoagulant, placed 30min ~ 40min at room temperature, was collected in the middle layer of yellowish white blood cells, each tube
Aliquot 500μL, 4000r/min centrifugal 10min, supernatant was removed and stored at -80 ℃ leukocyte precipitate.
4.1.3 Cell Culture
Prepared in accordance with Appendix A donkey in peripheral blood leukocytes, cultured cells were added to the growth solution 1d ~ 2d after, such as adherent cells have been extended and projections that
Disease can be inoculated material.
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