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SN/T 2713-2010: Quarantine protocol for Marteilia refringens in shellfish
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2713-2010 | English | 389 |
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Quarantine protocol for Marteilia refringens in shellfish
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SN/T 2713-2010
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PDF similar to SN/T 2713-2010
Standard similar to SN/T 2713-2010 GB 5009.261 SN/T 5662 SN/T 5480 SN/T 5195 Basic data | Standard ID | SN/T 2713-2010 (SN/T2713-2010) | | Description (Translated English) | Quarantine protocol for Marteilia refringens in shellfish | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B50 | | Word Count Estimation | 15,156 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682 | | Adopted Standard | OIE " Aquatic Animal Disease Diagnosis Handbook" (2009 edition), NEQ | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the refractive Marteilia quarantine technical specifications. This standard applies to refractive Marteilia Isolation and identification, but also for the disease epidemiology, diagnosis, quarantine and monitoring. | SN/T 2713-2010: Quarantine protocol for Marteilia refringens in shellfish---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for Marteilia refringens in shellfish
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Marr insect quarantine shellfish too technical specifications
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is not equivalent to the International Organization for Animal Health (OIE) "aquatic animal disease diagnostic manual" (2009 edition) 2.4.4 and other relevant
chapter.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine Xiamen People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Yue Zhiqin, Fangshao Qing, Deng Mingjun, German Fan, Wushao Jiang, Zhao Ran, Zheng Xiaolong, Liang beads, Xushu Philippines,
Xu Biao, Zhu China, Xiao Xi Zhi.
Marr insect quarantine shellfish too technical specifications
1 Scope
This standard specifies the technical specifications Marteilia refringens quarantine pest.
This standard applies to refractive isolation and identification of Malta too insects, but also for disease epidemiology, diagnosis, quarantine and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 equipment and materials
3.1 device
3.1.1 ordinary optical microscope.
3.1.2 tissue grinder.
3.1.3 slicer.
3.1.4 PCR instrument.
3.1.5 gel imager or ultraviolet light observation.
3.2 Material
3.2.1 Water. When tissue sections with 75% saline water. When do PCR used to use ultra-pure water. Water line with GB/T 6682
Provisions.
3.2.2 clean coverslip, slides (several).
3.2.3 coated with amino alkyl silane (aminoalkylsilane) slides adhesion agent.
3.2.4 stain. Conventional smears and histological stain (see Appendix A).
3.2.5 phenol - chloroform extraction method or other method of DNA extraction of tissue DNA extraction kit (see Appendix A).
3.2.6 proteinase K.
3.2.7 magnesium sulfate (MgSO4).
3.2.8 dNTP.
3.2.9 TaqDNA polymerase and PCR buffer.
3.2.10 DNADL2000Marker.
3.2.11 nucleic acid dye -EB (or new alternative dyes such as. GENEFINDER).
3.2.12 Bismarck brown (bismarckbrownyelow) dye.
3.2.13 running buffer (see Appendix A).
3.2.14 Primer
Pr4. 5'-CCG-CAC-ACG-TTC-TTC-ACT-CC-3 '
Pr5. 5'-CTC-GCG-AGT-TTC-GAC-AGA-CG-3 '
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