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SN/T 2709-2010: Detection of Clostridium perfringens toxins at frontier port
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| SN/T 2709-2010 | English | 259 |
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Detection of Clostridium perfringens toxins at frontier port
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SN/T 2709-2010
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Basic data | Standard ID | SN/T 2709-2010 (SN/T2709-2010) | | Description (Translated English) | Detection of Clostridium perfringens toxins at frontier port | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 10,188 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 4789.13; GB/T 4789.28; SN 0177; WS/T 7 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Clostridium perfringens food poisoning sample collection and transportation, Clostridium perfringens shuttle seedlings were isolated and cultured, Clostridium perfringens enterotoxin detection of Clostridium perfringens ��, �� and intestinal toxin genes by PCR and multiplex PCR and bio- safety requirements. This standard applies to border crossings and border ports of entry and exit personnel engaged in food production and service units practitioners Clostridium perfringens food poisoning, but also applies to the border port range of water, food, samples of Clostridium perfringens food poisoning clostridial toxin detection. | SN/T 2709-2010: Detection of Clostridium perfringens toxins at frontier port---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of Clostridium perfringens toxins at frontier port
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Border crossings Clostridium perfringens toxin detection method
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, People's Republic of China Zhuhai Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. phase Dapeng, SHI Yong Xia Lin Jican, Li Xiaobo, Chen Yonghong, Hong Ye, Huang Ji City, Kui Cheng, Xing Lu Qin, Gobo rotation,
Zhongyu Qing.
Border crossings Clostridium perfringens toxin detection method
1 Scope
This standard specifies the collection and delivery of Clostridium perfringens food poisoning samples, Clostridium perfringens were isolated and cultured, Clostridium perfringens
Detection of Clostridium, Clostridium perfringens α, PCR detection of β and toxin genes and multiplex PCR assay and biosafety enterotoxin
Claim.
This standard applies to border crossings and border ports of entry and exit personnel engaged in food production and service units of employees perfringens shuttle
Bacterial food poisoning, but also for the detection of Clostridium perfringens toxin border crossings range of water, food, food poisoning samples.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 4789.13 Microbiological examination of food hygiene Clostridium perfringens test
GB/T 4789.28 Microbiological examination of food hygiene staining, media and reagents
Clostridium perfringens test methods produce food for export SN 0177
WS/T 7 Clostridium perfringens food poisoning diagnostic criteria and principles
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Clostridium perfringens Clostridiumperfringens
Is a Gram-positive, produce spores, and strictly anaerobic Clostridium form a special capsule. Widely distributed in nature, water,
Present in the soil and in the gut of humans and animals, humans and animals can cause necrotizing enterocolitis, intestinal toxemia and drinking traumatic gas gangrene,
In particular cause severe food poisoning.
3.2
Clostridium perfringens toxins Clostridiumperfringenstoxins
Clostridium perfringens is mainly produced α, β, ε, τ exotoxin and enterotoxin CPE. According bacteria produce lethal α, β, ε, τ can exotoxin
Power can be classified into A ~ E 5 inoculum type, in which the main human pathogenic type A (α produce toxins, some strains also produce enterotoxin
CPE), there are a small number of C-type (produced α and β toxin toxins), the main cause human food poisoning and necrotizing enterocolitis, or even cause intestinal
Toxemia, cause death.
4 Reagents and materials
4.1 The main instruments
4.1.1 PCR amplification.
4.1.2 scales.
4.1.3 clean bench.
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