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Determination of microcystin in fresh water products for import and export. Enzyme-linked immunosorbent assay
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SN/T 2678-2010
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Basic data | Standard ID | SN/T 2678-2010 (SN/T2678-2010) | | Description (Translated English) | Determination of microcystin in fresh water products for import and export. Enzyme-linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 9,919 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of fish, shrimp, shellfish and other fresh products microcystin enzyme-linked immunosorbent assay method. This standard applies to export fish, shrimp, shellfish and other freshwater products in the detection of microcystins. | SN/T 2678-2010: Determination of microcystin in fresh water products for import and export. Enzyme-linked immunosorbent assay
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Determination of microcystin in fresh water products for import and export.Enzyme-linked immunosorbent assay
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Importers and freshwater products Microcystins
Detection ELISA
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine Qingdao, People's Republic of China
Bureau, Chinese Academy of Sciences Institute of Hydrobiology, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Wu Zhenxing, only Li Wei, Lu Qing, Gannan Qin, Song Lirong, Chen Wei, Cao occasion Juan, Wangqiu Yan.
Importers and freshwater products Microcystins
Detection ELISA
1 Scope
This standard specifies the ELISA detection method and export fish, shrimp, shellfish and other freshwater products of microcystin.
This standard applies to the import and export of fish, shrimp, shellfish and other freshwater products in the detection of microcystin.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
This standard aqueous methanol to extract freshwater products microcystin, after solid phase extraction (SPE) process, sample microcapsule
Algal toxin microcystin enzyme markers common competitive binding antibody previously coated on the microplate, repeated washing liquid to clean all pores, and then
After addition of chromogenic substrate, in order to then determined by comparing the absorbance values of the absorbance of the sample is worth microcystin content.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.1 microcystin detection kit (see Appendix A).
4.2 methanol.
4.3 Sodium hydroxide (NaOH).
4.4 Disodium hydrogen phosphate (Na2HPO3 · 12H2O).
4.5 extract. 70% aqueous methanol.
4.6 Eluent. 20% methanol aqueous solution.
4.7 Sodium hydroxide solution. Weigh sodium hydroxide (4.3) 0.4g, dissolved in water and dilute to 100mL.
4.8 phosphate buffer solution. Weigh disodium hydrogen phosphate (Na2HPO3 · 12H2O) (4.4) 34g, dissolved in water and dilute to 1L, hydroxide
Sodium hydroxide solution (4.7) adjusted to pH 8.0.
4.9 microcystin standards. purity greater than or equal to 95%.
4.10 microcystin standard stock solution. methanol as solvent formulated as a stock solution of 100μg/L, and stored at -20 ℃ condition.
4.11 SPE. SEP-PAKCLASSICC18 (or equivalent).
5. Apparatus
5.1 Balance. a sense of the amount of 0.01g.
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