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Determination of amnesic shellfish poison in shellfish. Enzyme-linked immunosorbent assay
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SN/T 2663-2010
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Basic data | Standard ID | SN/T 2663-2010 (SN/T2663-2010) | | Description (Translated English) | Determination of amnesic shellfish poison in shellfish. Enzyme-linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 7,710 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the amnesic shellfish and their products in the shellfish toxin testing ELISA. This standard applies to products in the amnesic shellfish and shellfish toxin testing. | SN/T 2663-2010: Determination of amnesic shellfish poison in shellfish. Enzyme-linked immunosorbent assay
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Determination of amnesic shellfish poison in shellfish.Enzyme-linked immunosorbent assay
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Amnesiac shellfish shellfish poison test methods
Enzyme-linked immunosorbent assay
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Yunnan People's Republic of China
Bureau, People's Republic of China Shandong CIQ.
The main drafters of this standard. Wangqiu Yan, Cao occasion Juan, Wang Gang, Zhao Xin, Zheng Huifang, the soldiers, Huang Daliang, Wu Zhenxing, Vega Xu Cheng Chiu,
Xu Junyi.
Amnesiac shellfish shellfish poison test methods
Enzyme-linked immunosorbent assay
1 Scope
This standard specifies the shellfish and their products amnesiac shellfish toxin ELISA test.
This standard applies to shellfish and products amnesiac shellfish toxin testing.
2 terms, definitions and abbreviations
2.1 Terms and Definitions
The following terms and definitions apply to this document.
2.1.1
Amnesiac shellfish poisoning amnesicshelfishpoison; ASP
Chemical structure represented by domoic acid, after ingestion can produce dizziness, disorientation and persistent short-term memory loss and other symptoms
Shellfish in general present in marine organisms of toxic substances, it is a class of excitatory amino acid substances have nerves.
2.1.2
Domoic acid domoicacid; DA
One kind of glutamic acid is mainly composed of diatoms genus Pseudo-nitzschia produced and red alginic acid analogs can contaminate fish, shellfish and other marine life, caused
Human amnesic poisoning causative factor.
2.2 Acronyms
The following abbreviations apply to this document.
ASP (amnesicshelfishpoinson). amnesiac shellfish poisoning.
ELISA (enzymelinkedimmunosorbentassay). enzyme-linked immunosorbent assay.
3 sample storage and preparation
Save 3.1 sample
3.1.1 Samples should be fully representative, and not as individual differences, and at least 10 more. Shelled shellfish, shelled or canned
Etc., shall take sufficient number and shellfish of 200g or more.
3.1.2 away from the laboratory not timely submission of samples, in addition to quality will not change at room temperature, samples should be placed in incubation box
Frozen inspection. As for the shell samples shall be 3.2 methods open the shell, after removal of the water freezing inspection.
3.2 Preparation of the sample
3.2.1 Pre-treatment samples of fresh shell
The outer shell with water thoroughly washed, cut off the adductor muscle, open the shell, rinsed with distilled water and heavy internal remove sediment and other foreign matter. will
Adductor muscle and tissue glue connecting the unit separately, carefully remove the shellfish meat, not cut flesh. Do not heat or open anesthetic front case. collect
100g shellfish meat is placed in a sieve draining 5min (Do not allow the accumulation of meat), and the detection of broken shells and other debris, the shellfish meat homogeneous set aside.
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