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SN/T 2627-2010: Detection and identification of potato leafroll virus for quarantine
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2627-2010 | English | 259 |
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Detection and identification of potato leafroll virus for quarantine
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SN/T 2627-2010
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Basic data | Standard ID | SN/T 2627-2010 (SN/T2627-2010) | | Description (Translated English) | Detection and identification of potato leafroll virus for quarantine | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,123 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry plant quarantine potato leafroll virus detection and identification procedures. This standard applies to Ma Qian potato tubers, seedlings and other Solanaceae potato leaf roll virus infected horse seal inspection and identification. | SN/T 2627-2010: Detection and identification of potato leafroll virus for quarantine---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of potato leafroll virus for quarantine
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quarantine and identification of potato leafroll virus
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Sichuan Exit Inspection and Quarantine, Plant Quarantine Station of Sichuan Agricultural Department, Sichuan Agriculture
the University.
The main drafters of this standard. Meng Xing, Ning Hong, Feng Zhang, Zhang Min, Tan Jiaxing Guo Jin Di, Wu Jie.
Quarantine and identification of potato leafroll virus
1 Scope
This standard specifies the entry plant quarantine potato leaf roll virus detection and identification procedures.
This standard applies to potato tubers, seedlings and other solanaceous plants infected potato leaf roll virus testing and identification.
Principle 2
Potato leafroll virus (potatoleafrolvirus, PLRV). Genus potato luteoviridae, potato leaf roll virus genus. virus
Mitochondria spherical particle diameter of 23nm ~ 25nm, is an isometric symmetry virus. Lethal temperature 70 ℃, dilution end point of about 10-4. Causing potato
Leaf curl virus disease. Potato virus disease is generally based on the symptoms of disease is difficult to determine the type of virus, using immunological and molecular biology methods can be fast
Potato leafroll virus quarantine speed were identified according to the relevant criteria.
3 Reagents and materials
Unless otherwise indicated, this standard uses only recognized analytical grade and distilled or deionized water or equivalent purity.
3.1 immunological test kits
3.1.1 capture antibody (Captureantibody) potato leaf roll virus immunoglobulin antibody dilution according to the requirements of a commercial product.
Stored at 4 ℃ for use.
3.1.2 HRP (Alkalinephosphataseenzymeconjugate). with alkaline phosphatase-labeled potato leaf roll virus for Free
Immunoglobulin antibody. According to the requirements of the commercially available product is diluted, store at 4 ℃ for use.
3.1.3 coating buffer (Coatingbuffer). Take 2.93g sodium bicarbonate (NaHCO3), 1.59g of sodium carbonate (Na2CO3), 0.2g
Sodium azide (NaN3), with 1000mL dissolved in distilled water, and adjusted to pH 9.6, stored at 4 ℃ for use.
3.1.4 washing solution (PBSTBuffer). Take 1.15g of anhydrous disodium hydrogen phosphate (Na2HPO4), 0.2g potassium chloride (KCl), 0.2g of anhydrous
Potassium dihydrogen phosphate (KH2PO4), 8.0g sodium chloride (NaCl), 0.5g Tween-20, with 1000mL dissolved in distilled water, and adjusted to pH
7.4.
3.1.5 Sample extract (GEBbuffer). Take 1.3g anhydrous sodium sulfate (Na2SO4), 20.0g of polyvinylpyrrolidone
[(C6H9NO) 24 ~ 40000], 0.2g sodium azide (NaN3), 2.0gⅡ level egg albumin powder, 20.0 g of Tween-20, with 1000mL
Washing liquid to dissolve, and adjust the pH to 7.4, stored at 4 ℃ for use.
3.1.6 HRP dilution buffer (ECIBuffer). Take 0.2g of bovine serum albumin (or skim milk), 2.0g polyvinylpyrrolidine
-one [(C6H9NO) n], 0.02g sodium azide (of NaN3), washed with 100mL buffer solution, and adjusted to pH 7.4, stored at
4 ℃ under the conditions of use.
3.1.7 substrate buffer (PNPBuffer). 80mL sterile distilled water with 0.01g of magnesium chloride (MgCl2), 0.02g sodium azide
(NaN3), after 9.7mL dihexyl amine alcohol (CH2CH2OH) was dissolved with hydrochloric acid adjusted to pH 9.8, made up to 100mL, stored at 4 ℃
Conditions for use.
3.1.8 substrate solution (PNPsubstate). The 5mg of p-nitrophenyl phosphate (C6H5NO3) was dissolved in 5mL of substrate buffer.
Preparation of substrate solution required within 15min, prepared in the absence of light before the end of incubation.
3.1.9 Stop Solution. 12g of sodium hydroxide (NaOH) was dissolved in 100mL of distilled water.
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