SN/T 2617: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2617-2022 | English | RFQ |
ASK
|
3 days [Need to translate]
|
(Quarantine and Identification Method of Winter Phytophthora)
| Valid |
SN/T 2617-2022
|
| SN/T 2617-2010 | English | 479 |
Add to Cart
|
3 days [Need to translate]
|
Detection and identification of Phytophthora hibernalis Carne
| Obsolete |
SN/T 2617-2010
|
PDF similar to SN/T 2617-2022
Basic data | Standard ID | SN/T 2617-2022 (SN/T2617-2022) | | Description (Translated English) | (Quarantine and Identification Method of Winter Phytophthora) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.20 | | Date of Issue | 2022-07-07 | | Date of Implementation | 2023-02-01 | | Issuing agency(ies) | General Administration of Customs | SN/T 2617-2010: Detection and identification of Phytophthora hibernalis Carne---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phytophthora hibernalis Carne
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Winter Health quarantine and identification of Phytophthora
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine.
Drafters of this standard. Wu Shan goods, Du Hongzhong, Yan Jin.
Winter Health quarantine and identification of Phytophthora
1 Scope
This standard specifies the phytosanitary born in winter Phytophthora PhytophthorahibernalisCarne quarantine authentication methods.
This standard applies to pathogen detection for Winter Health Phytophthora host seedlings, branches, leaves, fruits and entrained soil winter green Phytophthora
Phytophthora identification.
Principle 2
According to Winter Health Phytophthora symptoms on the host, morphological characteristics and PCR amplified fragment was identified specific basis. The bacteria which
He related information, see Appendix A.
3 Instrument
3.1 biological microscope, stereo microscope.
3.2 Light biological incubator.
3.3 autoclave.
3.4 high-speed centrifuges.
3.5 PCR amplification, electrophoresis, gel imager.
4 reagents, materials and media
4.1 Reagents
4.1.1 horses vancomycin, rifampin sodium, benomyl, hymexazol, calcium carbonate (CaCO3), β- sitosterol, tryptophan, sulfur, ammonia, calcium chloride
(CaCl2 · H2O), sodium hypochlorite (NaClO).
4.1.2 Agarose, of SDS (sodium dodecyl sulfate), Tris-HCl, magnesium chloride, hydrochloric acid, EDTA (ethylenediaminetetraacetic acid tetrasodium salt), hydroxide
Sodium acetate, sodium chloride, Tris, CTAB (cetyl trimethyl ammonium bromide), TAE, ethanol, phenol, chloroform, isopropanol,
Iso-amyl alcohol, SYBRGreenⅠ nucleic acid dyes.
4.1.3 proteinase K, TaqDNApolymerase, dNTP, DNA molecular weight Marker.
4.1.4 PCR primers PHIB1/PHIB2.
4.2 Material
Agar, V8 liquid, cedar (Cedrusdeodara) pine needle, citrus (Citrusspp.) Blade, carrot, corn oil.
4.3 Medium
The standard medium used, see Appendix B.
|