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Detection and identification of Clavibacter michiganensis subsp. michganensis (Smith) Jenen
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SN/T 2568-2010
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Basic data | Standard ID | SN/T 2568-2010 (SN/T2568-2010) | | Description (Translated English) | Detection and identification of Clavibacter michiganensis subsp. michganensis (Smith) Jenen | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 13,162 | | Date of Issue | 2010-05-28 | | Date of Implementation | 2010-12-01 | | Quoted Standard | GB 6682; SN/T 1538.1; SN/T 1809 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the tomato bacterial canker [Clavibactermichiganensis subsp. michiganensis (Smith) Davisetal] quarantine identification. This standard applies to the entry and exit of tomato seeds and their products in tomato bacterial canker quarantine and identification. | SN/T 2568-2010: Detection and identification of Clavibacter michiganensis subsp. michganensis (Smith) Jenen
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Detection and identification of Clavibacter michiganensis subsp.michganensis (Smith) Jenen
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Tomato bacterial canker quarantine and identification methods
michganensis (Smith) Jenen
Issued on. 2010-05-28
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xinjiang People's Republic of China Entry-Exit Inspection and Quarantine of Gansu People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine, People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters. Zhang Xianglin, Liu Qing, Wang, Zhao Wenjun, Wangyou Fu.
Tomato bacterial canker quarantine and identification methods
1 Scope
This standard specifies the tomato bacterial canker [Clavibactermichiganensissubsp.michiganensis (Smith)
Davisetal] quarantine authentication methods.
This standard applies to the entry and exit of tomato seeds and tomato products of bacterial canker quarantine and identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB 6682 Analysis
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1809 export plant quarantine rules for seed
Principle 3
Taxonomic status of tomato bacterial canker belonging to Firmicutes (Firmicutes), Firmicutes Gang (firmibacteria), eubacteria Head
(Enterobacteriaceae), Rod genus (Clavibacter). The biological characteristics of bacteria (see Appendix A), serological properties and points
Biological characteristics of the child is the main basis for the development of this standard.
4 equipment and appliances
4.1 Equipment
Electronic balance (inductance 1/1000g), vacuum pump, incubator, temperature oscillation incubator, autoclave, clean benches, biological
Microscope (with camera system), high-speed refrigerated centrifuge, Mini personal centrifuges, water, a constant temperature water bath, low temperature refrigerators, microplate reader, PCR
Instrument, electrophoresis, electrophoresis tank, gel imaging analyzer.
4.2 Appliances
Petri dishes, test tubes, adjustable micro-injector (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL) and the corresponding Injector
Head, mortar, enzyme-linked 96 hole reaction plate, centrifuge tubes (1.5mL, 10mL), PCR tubes (0.2mL), graduated cylinder, beaker.
5 Reagents
Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. It shall comply with GB 6682 test water in a water specifications.
Culture, identification of tomato bacterial canker reagents used in Appendix B, Appendix C and Appendix D.
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