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SN/T 2519-2010 English PDF

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SN/T 2519-2010: Determination of astrovirus in shellfish. Conventional RT-PCR and real-time RT-PCR method
Status: Valid
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SN/T 2519-2010English369 Add to Cart 3 days [Need to translate] Determination of astrovirus in shellfish. Conventional RT-PCR and real-time RT-PCR method Valid SN/T 2519-2010

PDF similar to SN/T 2519-2010


Standard similar to SN/T 2519-2010

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Basic data

Standard ID SN/T 2519-2010 (SN/T2519-2010)
Description (Translated English) Determination of astrovirus in shellfish. Conventional RT-PCR and real-time RT-PCR method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X20
Classification of International Standard 67.120.30
Word Count Estimation 14,196
Date of Issue 2010-03-02
Date of Implementation 2010-09-16
Quoted Standard GB/T 6682; GB 19489; SN/T 1193
Regulation (derived from) National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the shellfish Astroviruses ordinary RT-PCR and real-time fluorescent RT-RCR detection methods. This standard applies to shellfish Astroviruses detection, poisoning samples Astroviruses detection can refer to use.

SN/T 2519-2010: Determination of astrovirus in shellfish. Conventional RT-PCR and real-time RT-PCR method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of astrovirus in shellfish.Conventional RT-PCR and real-time RT-PCR method Exit inspection and quarantine industry standard book People's Republic of China Shellfish astrovirus detection method Common PCR and real-time PCR method Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Appendix A of this standard is a normative appendix, Appendix B and Appendix C are informative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine CNCA Certification and Accreditation Institute of Technology, National General Administration of Quality Supervision, Inspection and Quarantine standards and regulations, Beijing Ginna letter biotechnology company. The main drafters of this standard. Zeng Jing, Wei Haiyan, Yang Fan love red, red Rao, Jiang Ming, NIE edge, Zhang Meng West. This standard is the first release of the entry-exit inspection and quarantine industry standards. Shellfish astrovirus detection method Common PCR and real-time PCR method

1 Scope

This standard specifies the general shellfish astrovirus RT-PCR and real-time RT-PCR detection method. This standard applies to shellfish astrovirus detection; detection of food poisoning in the sample can refer to the use of astrovirus.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety SN/T 1193 genetic testing laboratory technical requirements 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this standard. 3.1 Terms and Definitions 3.1.1 Belonging Astroviridae, astrovirus genus. Virus particles are spherical, no envelope, diameter of 28nm ~ 30nm. Viral genome is a single Positive-strand RNA, long 6.8kb, 3 'polyA tail end there. 3.1.2 When the number of cycles the fluorescence signal of each reaction tube reaches the set threshold experienced. 3.1.3 The use of nanotechnology will be made of magnetic material such as Fe3O4 nanoparticles, the size of fine particles is generally between clusters and between (general diameter Less than 100nm), the quantum size effect MNP make it exhibit many unique properties, such as the large surface area, surface-active multi-center, table Surface reactivity, strong adsorption capacity, biological and medical applications for the research provides a more scientific means. 3.2 Acronyms 3.2.1 Coke ethylene carbonate. 3.2.2 Nano-magnetic beads.

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