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SN/T 2497.8-2010: Test method of import and export dangerous chemicals. Part 8: Popliteal lymph node assay
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| SN/T 2497.8-2010 | English | 139 |
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Test method of import and export dangerous chemicals. Part 8: Popliteal lymph node assay
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SN/T 2497.8-2010
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Basic data | Standard ID | SN/T 2497.8-2010 (SN/T2497.8-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 8: Popliteal lymph node assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 4,494 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Quoted Standard | GB 14924.1 | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals trials popliteal lymph node assay test principle, test methods and test results. This standard applies to the import and export of dangerous chemicals trials popliteal lymph node assay. | SN/T 2497.8-2010: Test method of import and export dangerous chemicals. Part 8: Popliteal lymph node assay
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Test method of import and export dangerous chemicals.Part 8. Popliteal lymph node assay
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 8. fossa lymph node test
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This section SN/T 2497 Part 8.
The partial modification of the use of "Toxicology test methods and techniques" immune toxicology research method, technical content and the method described above
Exactly the same, in a standard text format according to GB/T 1.1-2000 made editorial changes.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Lining Tao, Wangli Bing, in Yanjun, Zhao Zhuo, Zhang Yuan, Li Xue Yang.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 8. fossa lymph node test
1 Scope
This section SN/T 2497 specifies the test principle and export of dangerous chemicals test fossa lymph node assay, test methods
And test results.
This section applies to the import and export of dangerous chemicals Test fossa lymph node assay.
2 Normative references
The following documents contain provisions which, through reference SN/T 2497 to the present, constitute provisions of this section. For dated references,
All subsequent amendments (excluding corrections) or revisions do not apply to this section, however, encourage an agreement under this section
Parties to study the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
GB 14924.1 animal feed common quality standards
3 Test principle
The cause hypersensitivity or autoimmune response chemicals mice injected paw skin, causing internal fossa lymph node T, B cell proliferation
Leaving the lymph nodes, weight gain and change in cell number.
Test Method 4
4.1 test animals
Select (6-8) week old mice, the weight (18 ~ 22) g. Experimental animals and animal testing environment and facilities should meet the GB 14924.1 phase
The relevant requirements.
4.2 Test Supplies
Micro syringe, a 24-well culture plate, blood cell count boards, scales, microscopes.
4.3 test substance
The appropriate concentration of the sample dubbed, will not produce cytotoxicity, generally < 10mg/kg.
4.4 Test procedure
4.4.1 sensitization
The test solution in mice injected subcutaneously on the right hind paw 10μL from heel to toe direction, while the left hind paw subcutaneously
As a control 10μL injection solvent.
4.4.2 sacrificed
After continuous injection of 7d, mice were sacrificed, respectively, whichever is the hind limbs fossa lymph nodes (PLN), removing the surrounding connective tissue and fat weighed.
4.4.3 cell count
The nest filled with lymph node placed inside 1mL/saline bore 24-well plates, and made into a single cell suspension, counted the number of cells.
5 results
--- End point was PLN PLN cell mass index and index.
--- PLN mass index = weight of the test side PLN/side PLN weight control.
--- PLN cells test = index number of cell side PLN/PLN cells control side.
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