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SN/T 2497.27-2010: Test method of import and export dangerous chemicals. Part 27: The usage of flow cytometry in apoptosis
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2497.27-2010 | English | 159 |
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Test method of import and export dangerous chemicals. Part 27: The usage of flow cytometry in apoptosis
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SN/T 2497.27-2010
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Basic data | Standard ID | SN/T 2497.27-2010 (SN/T2497.27-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 27: The usage of flow cytometry in apoptosis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 6,666 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Adopted Standard | Toxicology experimental methods and techniques in the flow cytometry method, MOD | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals apoptosis by flow cytometry test terminology and definitions, test methods and analysis of results. This standard applies to import and export of dangerous chemicals apoptosis by flow cytometry test. | SN/T 2497.27-2010: Test method of import and export dangerous chemicals. Part 27: The usage of flow cytometry in apoptosis
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Test method of import and export dangerous chemicals.Part 27. The usage of flow cytometry in apoptosis
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 27. Flow Cytometry Apoptosis
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This part is Part 27 SN/T 2497 series of standards.
The partial modification of the use of "Toxicology test methods and technology" in the flow cytometry method of technical content and the method described above End
Full agreement on a standard text format according to GB/T 1.1 made editorial changes.
Appendix A of this section is an informative annex.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Chi Yu Feng, Wangli Bing, Wang Hua, Li Xue Yang, Liu Wei, Ding Yu.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 27. Flow Cytometry Apoptosis
1 Scope
SN/T 2497 This section specifies the terms and definitions import and export of hazardous chemicals flow cytometry apoptosis, test methods and conclusions
Analysis of fruit.
This section applies to the import and export of hazardous chemicals flow cytometry apoptosis test.
2 Terms and definitions
The following terms and definitions apply to this section.
2.1
Also known as "programmed cell death", a phenomenon of natural causes cells to environmental stimuli, gene regulation under produced. withered
Death was originally determined by characteristic morphological changes, including. procedural degradation of chromatin DNA concentration, cells shrink and crack. it can
Physiological or induced by the chemotherapy and radiation.
2.2
Vivo local tissue, cell death called necrosis. Metabolism necrotic tissue cells stop, loss of function. Morphological changes of necrosis may
So the damage caused by the degradation of intracellular hydrolase, the hydrolysis can also be caused by the migration of white blood cells to release enzymes.
2.3
Liquid is suspended cells or particles, physical properties and chemical properties, such as cell size, internal structure with flow cytometry measurements,
DNA, RNA, proteins, antigens for rapid determination of a kind of modern analytical techniques can be categorized collection.
Test Method 3
3.1 Equipment
--- Flow cytometry;
--- Ordinary low-speed centrifuge;
--- Vortex mixing apparatus;
--- Micro pipette.
3.2 Reagents and Consumables
--- AnnexinV/PI kit (containing fluorescently labeled AnnexinV, PI and BindingBuffer);
--- 200 mesh nylon filter mesh;
--- 0.22μm membrane;
--- PBS buffer (pH = 7.4) and distilled water (Filters for filtration);
--- Maintenance of flow cytometry reagents;
--- Pipette tip.
Reagents such as self-configuration, PBS and BingdingBuffer and PI configuration can be found in Appendix A.
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