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SN/T 2497.26-2010: Test method of import and export dangerous chemicals. Part 26: Determination of macrophage function
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Test method of import and export dangerous chemicals. Part 26: Determination of macrophage function
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SN/T 2497.26-2010
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Basic data | Standard ID | SN/T 2497.26-2010 (SN/T2497.26-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 26: Determination of macrophage function | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 5,562 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Quoted Standard | GB 14924.1; GB 14925 | | Adopted Standard | Immunology technology in the immune toxicology research methods, MOD | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals macrophage function test principle, test methods and test results. This standard applies to detect the import and export of dangerous chemicals immunotoxicity macrophage function test. | SN/T 2497.26-2010: Test method of import and export dangerous chemicals. Part 26: Determination of macrophage function
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Test method of import and export dangerous chemicals.Part 26. Determination of macrophage function
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 26. macrophage function tests
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This part is Part 26 SN/T 2497 series of standards.
The partial modification of the use of "Immunology technology" immune toxicology research method, technical content and the method described above exactly
Caused, in part, according to the text format GB/T 1.1 made editorial changes.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Li Jing, Wangli Bing, Wang Hua, Feng Yu-chi, chi in Rui Li Xue Yang.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 26. macrophage function tests
1 Scope
This section SN/T 2497.26 specifies the test principle and export of dangerous chemicals test macrophage function test, the test
Test methods and test results.
This section applies to detect the import and export of hazardous chemicals immunotoxicity macrophage function tests.
2 Normative references
The following documents contain provisions which, through reference SN/T 2497.26 of this part, constitute provisions of this part. For dated references
Documents, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Research into whether the parties to the agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB 14924.1 animal feed common quality standards
GB 14925 Laboratory Animal Facilities and Environment
3 Test principle
Macrophage cells as a primary mononuclear phagocyte system, with active phagocytosis. The body can eliminate antigenic substance and degeneration
Cells in specific and nonspecific immunity play an important role. Macrophage activation after stimulation by antigen, it may be obvious phagocytosis
Enhanced.
Test Method 4
4.1 test animals
BALB/C mice, ICR, Kunming strain mice, female or male, 20g ~ 22g. Experimental animal breeding conditions and should be consistent with GB 14924.1
GB 14925 of the relevant provisions.
4.2 Reagents
4.2.1 Candida albicans suspension. inoculated in Sabouraud medium Candida albicans, 28 ℃ cultured 18h ~ 24h, saline wash, dubbed
1 × 107 cells/mL cell suspension.
4.2.2 6% sterile starch.
4.2.3 Hank's solution (containing 5% fetal calf serum).
4.2.4 0.03% Meilan (4 ℃ storage).
4.3 Test procedure
4.3.1 Vivo
3 days prior to the test mice by intraperitoneal injection of 6% sterile starch solution 1mL, induce macrophages to the abdominal cavity exudate. During the test, each small
Mice by intraperitoneal injection of Candida albicans bacteria 1mL, gently massage the abdomen, so that a uniform distribution of bacteria in the abdominal cavity, is conducive to devour. 30min later, the mice
Pull were sacrificed, fixed, open the abdominal cavity exposed intestine, peritoneal fluid removed with ring vaccination, uniformly coated on a glass slide, and then a small drop
0.03% cold Meilan coverslip. High magnification observation, counting.
4.3.2 In vitro law
6% starch solution 1mL of sterilized mice by intraperitoneal injection. 72h later, the mice were sacrificed by cervical, abdominal, peritoneal fluid draw, 1000r/min
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