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SN/T 2497.16-2010: Test method of import and export dangerous chemicals. Part 16: Western-Blot
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2497.16-2010 | English | 139 |
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Test method of import and export dangerous chemicals. Part 16: Western-Blot
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SN/T 2497.16-2010
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Basic data | Standard ID | SN/T 2497.16-2010 (SN/T2497.16-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 16: Western-Blot | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 5,544 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals in Western-Blot test experimental terms and definitions, the main reagents, test procedures and test results. This standard applies to import and export of dangerous chemicals Western-Blot test. | SN/T 2497.16-2010: Test method of import and export dangerous chemicals. Part 16: Western-Blot---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method of import and export dangerous chemicals.Part 16. Western-Blot
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This is Part 16 SN/T 2497 series of standards.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Jia Xiaochuan, Wangli Bing, in Yanjun, Zhao Haolibao, Wang Hua, Xiong Zhongjiang.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
1 Scope
This section SN/T 2497 provisions of the import and export of dangerous chemicals in the Western-Blot test experimental terms and definitions, the main
Reagents, test procedures and test results.
This section applies to the import and export of dangerous chemicals test Western-Blot test.
2 Terms and definitions
The following terms and definitions apply to this section.
2.1
Cell or organism group commonly referred to as immunostaining or western blot, the basic principle is by specific antibody-treated gel electrophoresis
Tissue samples were colored. Achieve a particular protein in a cell or tissue analysis of the situation by analyzing the expression of the position of the colored and coloring depth
Status information.
2.2
To be tested as a counterpart antibody protein antigen.
2.3
Alkaline phosphatase-labeled antibody capable of binding with the first antibody.
3 Reagents
3.1 transfer buffer
Weigh glycine 2.9g, Tris base 5.8g, sodium dodecyl sulfate (SDS) 0.37g, add water to dissolve the solid, a solution of methanol
200mL, water to 1000mL.
3.2 10 × stock solution Ponceau S
Weigh Ponceau S2g, TCA 30g, sulfosalicylic acid 30g, dissolved in water, dilute to 100mL, stored at room temperature, diluted with time
10 times.
3.3 TBS solution
Weigh Tris base 3g, NaCl8g and 0.2gKCl, after dissolved in water, with dilute hydrochloric acid to adjust the pH value to 7.4, the volume to 1000mL,
In 1.034 × 105Pa (15lbf/in2) high-pressure steam sterilization 20min, stored at room temperature.
3.4 TBST solution
Press 1.1000 added in TBS Tween20, stored at room temperature.
3.5 alkaline phosphatase buffer
100mmol/LTris · Cl (pH value 9.5), 100mmol/LMgCl2,100mmol/LNaCl, stored at room temperature.
3.6 tetrazolium (NBT) stock solution
0.5gNBT 10mL70% dissolved in dimethyl formamide (DMF) in.
3.7 5-bromo-4-chloro-3-indolyl phosphate (of BCIP) stock solution
0.5gBCIP was dissolved in 10mL of dimethylformamide (DMF) in.
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