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Identification of Urocystis cepulae Frost
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SN/T 2464-2010
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Basic data | Standard ID | SN/T 2464-2010 (SN/T2464-2010) | | Description (Translated English) | Identification of Urocystis cepulae Frost | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,677 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 18085 | | Regulation (derived from) | National Quality Inspection (2010) No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry and exit of plant quarantine in the onion smut fungus (Urocystis cepulae) quarantine identification. This standard applies to the onion Allium host quarantine and identification of smut fungus. | SN/T 2464-2010: Identification of Urocystis cepulae Frost---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of Urocystis cepulae Frost
Exit inspection and quarantine industry standard book People's Republic of China
Article onion smut quarantine and identification of bacteria
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A and Appendix B are informative.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Liu Fang, Roca Feng, Guo Jingze, Zhang Yujun, Liuyue Ting, Cui Tiejun, Wang Jincheng, Liu Peng.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Article onion smut quarantine and identification of bacteria
1 Scope
This standard specifies the export plant quarantine onion strips smut fungus (Urocystiscepulae) of quarantine and identification methods.
This standard applies to Allium host quarantine and identification of the strips onion smut fungus.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 18085 phytosanitary wheat dwarf bunt quarantine and identification of bacteria
Principle 3
Article onion smut fungus (UrocystiscepulaeFrost) major damage Allium (Aliumspp.) Plants, plant Allium not harm other than
Thereof. Article on onion smut fungus host range, the ball spore morphology, host plants of symptoms (see Appendix A), onion agar spore
Bulb germination characteristics is based on the identification of the bacteria.
4 apparatus and appliances
Multifunctional Microscope (a micrometer), stereo microscope, hand-held magnifying glass, low-speed centrifuge, reciprocating oscillator, a vortex, dry heat
Sterilizers, autoclaves, general balance (a sense of volume 1/100g), biological safety cabinets or clean benches, biological incubator (with light), adjustable micropipette
Sampler (5μL ~ 200μL), flask (250mL), centrifuge tubes, slides (length × width 25.3mm × 76.2mm, thickness 0.8mm),
Coverslips (18mm × 18mm), Parafilm film or aluminum foil, Eppendorf tubes (1.5mL), Petri dishes, inoculating needle, triangle push.
5 Reagents and culture medium
5.1 Reagents
The active ingredient of 0.25% sodium hypochlorite solution, Tween -20 (Tween-20), Schiel's floating carrier (see GB/T 18085-2000
Appendix A Schiel's floating carrier preparation method), mounting medium, non-fluorescent microscope objective lens oil (Nd = 1.516), agar, sterile water.
5.2 Medium
Onion agar culture for pathogen save Characters Observation and pathogens.
Agar medium onion Preparation. Weigh agar powder 15g ~ 20g, was added 900mL of water and 100mL fresh healthy onion juice,
Load flask, 121 ℃ autoclave 30min, cooling to about 40 ℃, poured into a dish, after solidification, with triangle push dipped suspension
Uniformly coated surface of the medium.
6 laboratory tests
6.1 Inspection washed seeds
6.1.1 Sample preparation and centrifugation and volume
According to GB/T 18085 execution.
6.1.2 microscopy
Check the press GB/T 18085 pieces of onion smut spores ball Have Its shape and size, by checking for suspicious spores into a ball
Step for germination experiments.
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