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| SN/T 2462-2010 | English | 319 |
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Identification of cotton leaf curl Begomovirus
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SN/T 2462-2010
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Basic data | Standard ID | SN/T 2462-2010 (SN/T2462-2010) | | Description (Translated English) | Identification of cotton leaf curl Begomovirus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,859 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Regulation (derived from) | National Quality Inspection (2010) No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the cotton leaf curl virus (Cotton leaf curl Begomovirus, referred CLCuV) quarantine identification. This standard applies to possibly with cotton leaf curl virus in vivo host plant quarantine and identification. | SN/T 2462-2010: Identification of cotton leaf curl Begomovirus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of cotton leaf curl Begomovirus
Exit inspection and quarantine industry standard book People's Republic of China
Cotton leaf curl virus quarantine and identification methods
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Xinjiang People's Republic of China Exit Inspection and Quarantine, Zhejiang University, Chinese Academy of Inspection and Quarantine.
The main drafters. Zhang Xianglin, Zhou Xueping, water Zhu Fang, Wang large fishing net, Wei Wu, Yu Yongjie.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Cotton leaf curl virus quarantine and identification methods
1 Scope
This standard specifies the cotton leaf curl virus (CottonleafcurlBegomovirus, referred CLCuV) of quarantine and identification methods.
This standard applies to quarantine identify possible with cotton leaf curl virus in vivo host plants.
Principle 2
Taxonomic status of cotton leaf curl virus belonging to the virus family twin (Geminiviridae), bean golden mosaic virus genus (Begomovirus).
Biological characteristics of the virus, serological properties and molecular characterization is the main basis for the development of this standard (see Appendix A).
3 equipment and appliances
3.1 Equipment
Electronic balance (inductance 1/1000g), clean table, high-speed refrigerated centrifuge, small low-speed centrifuges, pH meter, water bath pot, micro
Amount juicer, ultra-low temperature freezer, autoclave, microplate reader, PCR instrument, electrophoresis, electrophoresis tank, gel imaging analyzer.
3.2 Appliances
Mortar, dish, adjustable micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL) and the appropriate pipette
Head, enzyme-linked 96-well plate, centrifuge tube (1.5mL, 10mL), PCR tubes (0.2mL), graduated cylinders, beakers forceps.
4 reagents, buffers and solutions
4.1 10 × PBST buffer
Potassium chloride (KCl) 2g, sodium chloride (NaCl) 80g, potassium dihydrogen phosphate (KH2PO4) 2g, disodium hydrogen phosphate (Na2HPO4 · 12H2O)
11.5g, Tween 20 (Tween-20) 5mL, adjusted to pH 7.4 with concentrated hydrochloric acid (HCl), distilled water and dilute to 1000mL, 4 ℃ condition
Under storage.
4.2 Sample Extraction buffer
Sodium sulfite (Na2SO3) 1.3g, polyvinyl pyrrolidone (MW24000 ~ 40000, PVP) 20g, sodium azide (of NaN3)
0.2g, Tween 20 (Tween-20) 20mL dissolved in 800mL1 × PBST, the pH was adjusted to 7.4 with hydrochloric acid, add 1 × PBST set
Volume to 1000mL, at 4 ℃ storage.
4.3 coating buffer
Sodium carbonate (Na2CO3) 1.59g, sodium bicarbonate (NaHCO3) 2.93g, sodium azide (NaN3) 0.2g, with concentrated hydrochloric acid (HCl) regulation
pH to 9.6, in distilled water and dilute to 1000mL, 4 ℃ storage conditions.
4.4 wash buffer
The 1 × PBST buffer adjusted to pH with concentrated hydrochloric acid (HCl) at 7.4,4 ℃ storage conditions.
4.5 HRP buffer
1 × PBST buffer 800mL, bovine serum albumin (BSA) 2g, polyvinylpyrrolidone 20g, sodium azide (NaN3)
0.2g, with sterile distilled water and dilute to 1000mL, at 4 ℃ storage.
4.6 substrate (PNPP) buffer
Diethanolamine 97mL, sodium azide (NaN3) 0.2g, with concentrated hydrochloric acid (HCl) to adjust the pH to 9.8 with sterile distilled water to volume
1000mL, at 4 ℃ storage.
4.7 substrate (PNPP) solution
The 4-nitrophenol sodium salt (PNPP) 100mg was dissolved in 100mL PNPP substrate buffer, and now with the current.
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