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SN/T 2416-2010: Detection method of staphylococal enterotoxin A in food. Electrophoretic and immunoblot method
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Detection method of staphylococal enterotoxin A in food. Electrophoretic and immunoblot method
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SN/T 2416-2010
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Basic data | Standard ID | SN/T 2416-2010 (SN/T2416-2010) | | Description (Translated English) | Detection method of staphylococal enterotoxin A in food. Electrophoretic and immunoblot method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 9,995 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 4789.10-2003; GB 19489 | | Adopted Standard | FDA BAM, MOD | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export food staphylococcal enterotoxin A electrophoresis and immunoblot detection methods. This standard applies to import and export food staphylococcal enterotoxin A detection. | SN/T 2416-2010: Detection method of staphylococal enterotoxin A in food. Electrophoretic and immunoblot method
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Detection method of staphylococal enterotoxin A in food.Electrophoretic and immunoblot method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food Staphylococcus aureus enterotoxin A
Detection electrophoresis and immunoblotting
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The standard reference American FDABAM Chapter 13 "Food Staphylococcus aureus enterotoxin A electrophoresis and immunoblotting"
(U.S.Food & DrugAdministrationCenterforFoodSafety & AppliedNutritionBacteriologicalAna-
lyticalManualOnlineJanuary2001Chapter13b. ElectrophoreticandImmunoblotAnalysisofStaphy-
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Fujian Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Guangdong Entry Inspection and Quarantine Bureau.
The main drafters of this standard. Wang Feng, Lidan Lin, Wang Xiaoyu, Tang food Ming Cheng Jing, Wu Zhaohui, tour Shu Chu, Chen Bin.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export food Staphylococcus aureus enterotoxin A
Detection electrophoresis and immunoblotting
1 Scope
This standard specifies the import and export food Staphylococcus aureus electrophoresis and immunoblotting detection of enterotoxin A.
This standard applies to imported and exported food staphylococcus enterotoxin A's.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 4789.10-2003 Microbiological examination of food hygiene inspection of Staphylococcus aureus
GB 19489 General requirements for laboratory biosafety
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
Protein exotoxin produced by Staphylococcus aureus is one of the main serotypes.
3.2
Specific Proteins were transferred onto a solid support using an antibody attached to a solid support occurred target protein epitopes presented
The reaction is detected.
Principle 4
SDS (sodium dodecyl sulphate) and the protein molecules to form a negatively charged protein -SDS complex. Under the influence of an electric field,
-SDS Protein complexes with different phases to the electrodes of the charge movement. Polyacrylamide gel according to molecular sieving, the migration rate of the complex
Negative correlation to its molecular weight. The electrophoretically separated components were transferred and fixed to a synthetic film after a solid support, a specific immune response
Blot analysis and color systems.
5 laboratory bio-safety requirements
5.1 The laboratory shall in accordance with GB 19489 Biosafety Level 2 (BSL-2) laboratory biosafety requirements for execution.
5.2 When possible when performing aerosol or liquid spilled operations, or may produce large amounts of toxins experiments, in addition to 5.1, but should also
Accordance with GB 19489 Dui BSL-3 personal protective equipment and safety requirements.
5.3 used in accordance with GB 19489 Lab Supplies shall Dispose of waste treatment and disposal.
6 Equipment and Materials
6.1 vertical gel system.
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