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Quarantine identification of tomato spotted wilt virus
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SN/T 2368-2009
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Basic data | Standard ID | SN/T 2368-2009 (SN/T2368-2009) | | Description (Translated English) | Quarantine identification of tomato spotted wilt virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 13,16 | | Date of Issue | 2009-09-02 | | Date of Implementation | 2010-03-16 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | Entry-Exit Inspection and Quarantine circular industry standards (2009. 09. 02) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry in the right phytosanitary quarantine tomato spotted wilt virus identification methods. This standard applies to all entry seeds, seedlings and other plant propagating material of tomato spotted wilt virus in quarantine and identification. | SN/T 2368-2009: Quarantine identification of tomato spotted wilt virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine identification of tomato spotted wilt virus
Exit inspection and quarantine industry standard book People's Republic of China
Tomato spotted wilt virus quarantine and identification methods
Posted 2009-09-02
2010-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix B, Appendix C, Appendix D, Appendix E are normative appendices, Appendix A is an information appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Liaoning Entry-Exit Inspection and Quarantine negative Tianjin People's Republic of China
Responsible for drafting.
The main drafters of this standard. Wang Xiufen, Li Jiang, Liu Wei, Liu Yueting, Wangyou Fu, Liushan Bin, Zhou Ming.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Tomato spotted wilt virus quarantine and identification methods
1 Scope
This standard specifies the entry in the phytosanitary tomato spotted wilt virus in quarantine and identification methods.
This standard applies to all entry quarantine and identification of seeds, seedlings and other plant propagation materials of tomato spotted wilt virus.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
Principle 3
3.1 scientific name and taxonomic status
Name. Tomatospottedwiltvirus
Abbreviation. TSWV
3.2 based quarantine and identification
Serological characteristics of tomato spotted wilt virus, properties and biological characteristics of molecular biology is the main basis for quarantine and identification of.
Additional information see Appendix A.
4 equipment, appliances, and reagents
4.1 Equipment
Enzyme-linked detector, the balance (a sense of volume, 1/10000g), pH meter, trace juicer, PCR instrument, electrophoresis, electrophoresis tank, gel imaging system,
Greenhouse isolation, constant temperature water bath, cold refrigerator.
Micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), ELISA plate, mortar, eppendorf tubes, flower pots, disinfection
Soil.
4.2 Reagents
Enzyme-linked immunosorbent assay reagent (see Appendix B), RT-PCR detection reagent (see Appendix C).
5 Sampling and sample preparation
5.1 Sampling
Sampling carried out in accordance SN/T 2122's.
5.2 Preparation of seed samples
Seeds (emphasis picked malformation, immature seed) sown in sterilized soil, grown and symptoms observed at 25 ℃. Be grown
3 to 4 leaves of plants will show symptoms numbers do not show symptoms of the plant grouping (10 per group) and number. Leaf collection for
Enzyme-linked immunosorbent assay, RT-PCR Detection and biological assay.
5.3 Preparation of the sample seedlings
The seedlings were grown in a greenhouse in isolation, growth and symptoms observed at 25 ℃. Symptomatic seedlings individually tested. No symptoms of sub
Group testing, grouping method and detection methods with 5.2.
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