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Import and export of milk and dairy products Tetracycline drug residue detection method radioreceptor analysis
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SN/T 2309-2009
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Basic data | Standard ID | SN/T 2309-2009 (SN/T2309-2009) | | Description (Translated English) | Import and export of milk and dairy products Tetracycline drug residue detection method radioreceptor analysis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100.01 | | Word Count Estimation | 15,193 | | Date of Issue | 2009-07-07 | | Date of Implementation | 2010-01-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection [2009] No. 290 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of milk and dairy products in the determination of tetracyclines residues Radioreceptor sample preparation and analytical methods. This standard applies to milk, milk powder, whey powder tetracycline, oxytetracycline, chlortetracycline, doxycycline drug residue screening tests. | SN/T 2309-2009: Import and export of milk and dairy products Tetracycline drug residue detection method radioreceptor analysis
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Import and export of milk and dairy products Tetracycline drug residue detection method radioreceptor analysis
Exit inspection and quarantine industry standard book People's Republic of China
Import and export milk and dairy products Tetracycline Residues
Radio-receptor assay detection method
Posted 2009-07-07
2010-01-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Lu Qin, Raymond Zhang dollars, Sunliang Juan, Li rights.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export milk and dairy products Tetracycline Residues
Radio-receptor assay detection method
1 Scope
This standard specifies the sample preparation and analysis methods radioreceptor import and export milk and dairy products Determination of residues of tetracyclines.
This standard applies to milk, milk powder, whey powder tetracycline, oxytetracycline, chlortetracycline, doxycycline screening to detect drug residues.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Sample preparation and preservation
3.1 Sample Preparation
Remove some representative samples from the original sample mix, milk powder, whey powder less than 500g, milk less than 500mL, sharing two
Parts, loaded into a clean container as a sample, and sealed and labeled. Sample preparation process should prevent sample contamination or microbial or chemical
The occurrence of the change of residue content.
3.2 Sample preservation
Dry samples were sealed and stored at room temperature, the liquid sample 0 ℃ ~ 4 ℃ refrigerator to save 5d, or less -15 ℃ can be stored frozen for two months.
4 Detection
4.1 Method summary
Based assay is a competitive receptor immunoreactivity. When the sample contains tetracycline antibiotics, tetracycline antibiotics residues in the sample
He was left with a binding site on receptor binding, thus preventing the [3H] labeled tetracycline receptor binding agent sites. Samples Sihuan
Su drug content is higher, the competition binding sites, [3H] tetracycline binding to the less marked, measured by scintillation counting with a liquid sample
Products in [3H] content cpm value (countperminute, that is, the number of pulses per minute) lower. Samples with residues of tetracyclines
cpm value is inversely proportional.
4.2 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.2.1 milk tetracycline antibiotics CharmⅡ radioimmunoassay kit (MRLsCompetitiveAssay) 1). pills identified as T.
1) CharmⅡ milk tetracyclines competitive receptor binding test kit is provided by the product under the trade name Charm company. Given this
Information in order to facilitate a user of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use
These equivalent.
4.2.1.1 receptor reagent tablets. the following -15 ℃ cryopreservation.
4.2.1.2 [3H] tetracycline tablets marked. the following -15 ℃ cryopreservation.
4.2.2 Negative control milk. proven antibiotic-free milk.
4.2.3 oxytetracycline standard.
4.2.4 scintillation fluid.
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