|
US$519.00 · In stock
Delivery: <= 4 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 2222-2008: Determination of glucocorticosteroids residues in foodstuffs of animal origin for import and export. LC-MS/MS method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2222-2008 | English | 519 |
Add to Cart
|
4 days [Need to translate]
|
Determination of glucocorticosteroids residues in foodstuffs of animal origin for import and export. LC-MS/MS method
| Valid |
SN/T 2222-2008
|
PDF similar to SN/T 2222-2008
Basic data | Standard ID | SN/T 2222-2008 (SN/T2222-2008) | | Description (Translated English) | Determination of glucocorticosteroids residues in foodstuffs of animal origin for import and export. LC-MS/MS method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 20,295 | | Date of Issue | 2008-11-18 | | Date of Implementation | 2009-06-01 | | Regulation (derived from) | National Quality Inspection [2008] No. 577 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of foodstuffs of animal origin triamcinolone, prednisolone, hydrocortisone, prednisone, dexamethasone, flumetasone, triamcinolone acetonide residue liquid chromatography mass spectrometry/mass spectrometry Methods. This standard applies to pork, kidney glucocorticoid Residues in the detection and confirmation. | SN/T 2222-2008: Determination of glucocorticosteroids residues in foodstuffs of animal origin for import and export. LC-MS/MS method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of glucocorticosteroids residues in foodstuffs of animal origin for import and export.LC-MS/MS method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export food of animal origin glucocorticoid
Detection of Residues in
Liquid chromatography - mass spectrometry/mass spectrometry
Posted 2008-11-18
2009-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix, Appendix B and Appendix C are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Liaoning Entry-Exit Inspection and Quarantine People's Republic of China, Zhejiang, China seized
Inspection and Quarantine Science Research Institute, Heilongjiang People's Republic of China Exit Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Linwei Xuan, Xie Kang celebrate, Xing right, Xiao Shanshan Tian Miao, Xijun Yang, in spirit.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export food of animal origin glucocorticoid
Detection of Residues in
Liquid chromatography - mass spectrometry/mass spectrometry
1 Scope
This standard specifies the import and export of animal-derived foods triamcinolone, prednisolone, hydrocortisone, prednisone, dexamethasone, betamethasone fluorine,
Triamcinolone acetonide Determination of Residual liquid chromatography - mass spectrometry/mass spectrometry methods.
This standard applies to pork, pig kidney glucocorticoid Residues in the detection and confirmation.
2 Method summary
First sample was added ammonium acetate buffer solution of hydrochloric acid and β- glucuronidase enzymes glycosides - aryl sulfatase hydrolysis, and then extracted with ethyl acetate extract
It was dried HLB SPE column purification, liquid chromatography - mass spectrometry/mass spectrometry and validation, external standard.
3 Reagents and materials
Unless otherwise specified, all reagents were of analytical grade, double distilled water.
3.1 acetonitrile. HPLC fractions.
3.2 hexane. HPLC fractions.
Ethyl acetate 3.3. HPLC grade.
3.4 Methanol. HPLC fractions.
3.5 ammonium acetate.
3.6 glacial acetic acid.
3.7 anhydrous sodium. 650 ℃ burning 4h, cooled in a desiccator to room temperature and stored in a sealed bottle aside.
3.8 β- hydrochloric acid glucuronide glycoside enzymes - arylsulfatase. hydrochloric acid containing β- glucuronidase enzyme glycosides 134600U/mL, arylsulfatase
5200U/mL.
3.9 Methanol - water (7 + 3, volume ratio) .30mL 70mL of methanol and water. Methanol - water (1 + 1, volume ratio).
3.10 methanol - water (5 + 5, volume ratio) .50mL 50mL of methanol and water.
3.11 0.02mol/L ammonium acetate buffer solution. 1.54g of ammonium acetate was dissolved in 950mL of water, the solution was adjusted to pH with glacial acetic acid
5.2, and finally diluted with water to 1L.
3.12 standard purity not less than 97%. Standard information, see Appendix A, Table A. 1.
3.13 glucocorticoid standard stock solution. Weigh the right amount of standard (3.12), dissolved in methanol formulated in a concentration of 100μg/mL standard
Quasi stock solution, -18 ℃ cryopreservation, valid for three months.
3.14 standard solution. According to the need to use a blank sample solution was diluted to a stock standard solution of dexamethasone concentration of 1,2,4, respectively,
6 and 8ng/mL, the concentration of prednisolone solution (pork) were 6,8,16,24 and 40ng/mL, triamcinolone, hydrocortisone, splash
Nison, Flumetasone, triamcinolone acetonide concentration of the mixed solution were 10,20,40,80 and 100ng/mL. Prior to use the existing service.
3.15 anhydrous sodium sulfate column. 80mm × 40mm (inner diameter) cylindrical funnel bottom pad 5mm cotton, refill 40mm anhydrous sodium sulfate.
3.16 SPE. Oasis (HLB) 500mg, or equivalent person.
The organic phase was 3.17 microporous membrane. 0.45μm.
4 instruments and equipment
4.1 High performance liquid chromatography - mass spectrometry/mass spectrometer. with electrospray ionization source.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2222-2008_English be delivered?Answer: Upon your order, we will start to translate SN/T 2222-2008_English as soon as possible, and keep you informed of the progress. The lead time is typically 2 ~ 4 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of SN/T 2222-2008_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 2222-2008_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|